Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3655872..3656503 Replicon chromosome
Accession NZ_CP012140
Organism Shigella flexneri 4c strain 1205

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag AD871_RS19890 Protein ID WP_001259384.1
Coordinates 3656228..3656503 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AD871_RS19885 Protein ID WP_000593555.1
Coordinates 3655872..3656231 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD871_RS19860 (3652004) 3652004..3652948 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
AD871_RS19865 (3652945) 3652945..3653778 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
AD871_RS19870 (3653778) 3653778..3654542 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
AD871_RS19875 (3654539) 3654539..3655345 + 807 WP_061456188.1 nickel import ATP-binding protein NikE -
AD871_RS19880 (3655351) 3655351..3655752 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AD871_RS19885 (3655872) 3655872..3656231 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AD871_RS19890 (3656228) 3656228..3656503 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
AD871_RS19895 (3656562) 3656562..3657686 - 1125 WP_001216257.1 ABC transporter permease -
AD871_RS19900 (3657686) 3657686..3660421 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
AD871_RS19905 (3660418) 3660418..3661485 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3655872..3675616 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T56017 WP_001259384.1 NZ_CP012140:c3656503-3656228 [Shigella flexneri 4c]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T56017 NZ_CP012140:c3656503-3656228 [Shigella flexneri 4c]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT56017 WP_000593555.1 NZ_CP012140:c3656231-3655872 [Shigella flexneri 4c]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT56017 NZ_CP012140:c3656231-3655872 [Shigella flexneri 4c]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References