Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3634449..3635080 Replicon chromosome
Accession NZ_CP012137
Organism Shigella flexneri 2a strain 981

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag AD867_RS19680 Protein ID WP_001259384.1
Coordinates 3634805..3635080 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AD867_RS19675 Protein ID WP_000593555.1
Coordinates 3634449..3634808 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD867_RS19650 (3630581) 3630581..3631525 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
AD867_RS19655 (3631522) 3631522..3632355 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
AD867_RS19660 (3632355) 3632355..3633119 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
AD867_RS19665 (3633116) 3633116..3633922 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
AD867_RS19670 (3633928) 3633928..3634329 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AD867_RS19675 (3634449) 3634449..3634808 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AD867_RS19680 (3634805) 3634805..3635080 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
AD867_RS19685 (3635139) 3635139..3636263 - 1125 WP_001216257.1 ABC transporter permease -
AD867_RS19690 (3636263) 3636263..3638998 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
AD867_RS19695 (3638995) 3638995..3640062 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3634449..3654193 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T55989 WP_001259384.1 NZ_CP012137:c3635080-3634805 [Shigella flexneri 2a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T55989 NZ_CP012137:c3635080-3634805 [Shigella flexneri 2a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT55989 WP_000593555.1 NZ_CP012137:c3634808-3634449 [Shigella flexneri 2a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT55989 NZ_CP012137:c3634808-3634449 [Shigella flexneri 2a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References