Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1627530..1628056 Replicon chromosome
Accession NZ_CP012126
Organism Escherichia coli strain DH1Ec104

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ADZ27_RS08045 Protein ID WP_000323025.1
Coordinates 1627530..1627817 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ADZ27_RS08050 Protein ID WP_000534858.1
Coordinates 1627817..1628056 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ27_RS08005 1622554..1622769 - 216 WP_000839590.1 phage lysis protein EssD -
ADZ27_RS08010 1623523..1623738 - 216 WP_000066484.1 cold shock-like protein CspB -
ADZ27_RS08015 1624039..1624251 + 213 WP_000087756.1 cold shock-like protein CspF -
ADZ27_RS25350 1624306..1624395 + 90 WP_120795389.1 hypothetical protein -
ADZ27_RS08020 1624673..1625425 - 753 WP_001047135.1 antitermination protein -
ADZ27_RS08025 1625439..1626488 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ADZ27_RS08030 1626490..1626768 - 279 WP_012304870.1 hypothetical protein -
ADZ27_RS08035 1626835..1627086 - 252 WP_000980994.1 hypothetical protein -
ADZ27_RS08040 1627303..1627458 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ADZ27_RS08045 1627530..1627817 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ADZ27_RS08050 1627817..1628056 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ADZ27_RS23540 1628081..1628386 + 306 WP_001326990.1 hypothetical protein -
ADZ27_RS08055 1628589..1628921 + 333 WP_001301033.1 protein FlxA -
ADZ27_RS22775 1629358..1629507 - 150 WP_011443592.1 hypothetical protein -
ADZ27_RS08060 1629542..1629820 - 279 Protein_1548 hypothetical protein -
ADZ27_RS08065 1629804..1630034 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ADZ27_RS08070 1630118..1630525 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ADZ27_RS08075 1630692..1630847 + 156 WP_000379575.1 DUF1391 family protein -
ADZ27_RS08080 1631007..1631225 + 219 WP_001171942.1 hypothetical protein -
ADZ27_RS08090 1631793..1631981 + 189 WP_000854559.1 cell division inhibition protein DicB -
ADZ27_RS08095 1631978..1632169 + 192 WP_001083297.1 lysis protein YdfD -
ADZ27_RS23555 1632262..1633029 + 768 Protein_1555 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1615806..1634892 19086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T55883 WP_000323025.1 NZ_CP012126:c1627817-1627530 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T55883 NZ_CP012126:c1627817-1627530 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT55883 WP_000534858.1 NZ_CP012126:c1628056-1627817 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT55883 NZ_CP012126:c1628056-1627817 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References