Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1627529..1628055 Replicon chromosome
Accession NZ_CP012125
Organism Escherichia coli strain DH1Ec095

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ADS71_RS08045 Protein ID WP_000323025.1
Coordinates 1627529..1627816 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ADS71_RS08050 Protein ID WP_000534858.1
Coordinates 1627816..1628055 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADS71_RS08005 1622553..1622768 - 216 WP_000839590.1 phage lysis protein EssD -
ADS71_RS08010 1623522..1623737 - 216 WP_000066484.1 cold shock-like protein CspB -
ADS71_RS08015 1624038..1624250 + 213 WP_000087756.1 cold shock-like protein CspF -
ADS71_RS25355 1624305..1624394 + 90 WP_120795389.1 hypothetical protein -
ADS71_RS08020 1624672..1625424 - 753 WP_001047135.1 antitermination protein -
ADS71_RS08025 1625438..1626487 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ADS71_RS08030 1626489..1626767 - 279 WP_012304870.1 hypothetical protein -
ADS71_RS08035 1626834..1627085 - 252 WP_000980994.1 hypothetical protein -
ADS71_RS08040 1627302..1627457 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ADS71_RS08045 1627529..1627816 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ADS71_RS08050 1627816..1628055 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ADS71_RS23550 1628080..1628385 + 306 WP_001326990.1 hypothetical protein -
ADS71_RS08055 1628588..1628920 + 333 WP_001301033.1 protein FlxA -
ADS71_RS22780 1629357..1629506 - 150 WP_011443592.1 hypothetical protein -
ADS71_RS08060 1629541..1629819 - 279 Protein_1548 hypothetical protein -
ADS71_RS08065 1629803..1630033 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ADS71_RS08070 1630117..1630524 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ADS71_RS08075 1630691..1630846 + 156 WP_000379575.1 DUF1391 family protein -
ADS71_RS08080 1631006..1631224 + 219 WP_001171942.1 hypothetical protein -
ADS71_RS08090 1631792..1631980 + 189 WP_000854559.1 cell division inhibition protein DicB -
ADS71_RS08095 1631977..1632168 + 192 WP_001083297.1 lysis protein YdfD -
ADS71_RS23565 1632261..1633028 + 768 Protein_1555 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1615805..1634891 19086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T55840 WP_000323025.1 NZ_CP012125:c1627816-1627529 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T55840 NZ_CP012125:c1627816-1627529 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT55840 WP_000534858.1 NZ_CP012125:c1628055-1627816 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT55840 NZ_CP012125:c1628055-1627816 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References