Detailed information of TA system TA055103

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3295190..3295405
• Replicon: chromosome
• Accession: NZ_CP011847
• Organism: Clostridioides difficile strain DSM 27639

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: CDIF27639_RS15525
• Protein ID: WP_011861731.1
• Coordinates: 3295262..3295405 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	3295190..3295336 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDIF27639_RS15510	3290849..3292003	+	1155	WP_003439713.1	GGDEF domain-containing protein	-
CDIF27639_RS15515	3292084..3293802	-	1719	WP_011861730.1	putative 2-aminoethylphosphonate ABC transporter permease subunit	-
CDIF27639_RS15520	3293807..3294688	-	882	Protein_2904	ATP-binding cassette domain-containing protein	-
-	3295190..3295336	+	147	NuclAT_0	-	Antitoxin
-	3295190..3295340	+	151	NuclAT_1	-	-
CDIF27639_RS15525	3295262..3295405	-	144	WP_011861731.1	hypothetical protein	Toxin
CDIF27639_RS15530	3295663..3295851	+	189	WP_009898401.1	hypothetical protein	-
CDIF27639_RS15535	3296098..3296802	-	705	WP_011861732.1	hypothetical protein	-
CDIF27639_RS15540	3296804..3297235	-	432	WP_011861052.1	protein-export chaperone SecB	-
CDIF27639_RS15545	3297253..3297816	-	564	WP_021371073.1	hypothetical protein	-
CDIF27639_RS15550	3298362..3298658	-	297	WP_011861049.1	hypothetical protein	-
CDIF27639_RS15555	3298675..3299490	-	816	WP_021362018.1	N-acetylmuramoyl-L-alanine amidase	-
CDIF27639_RS15560	3299487..3299747	-	261	WP_011861047.1	phage holin family protein	-
CDIF27639_RS15565	3299766..3299996	-	231	WP_021362019.1	hemolysin XhlA family protein	-
CDIF27639_RS20220	3300035..3300205	-	171	WP_021362020.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3290882..3349186	58304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T55103 WP_011861731.1 NZ_CP011847:c3295405-3295262 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T55103 NZ_CP011847:c3295405-3295262 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 147 bp

>AT55103 NZ_CP011847:3295190-3295336 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin