Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2897484..2898010 Replicon chromosome
Accession NZ_CP011495
Organism Escherichia coli strain NCM3722 isolate K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AA953_RS14440 Protein ID WP_000323025.1
Coordinates 2897484..2897771 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AA953_RS14445 Protein ID WP_000534858.1
Coordinates 2897771..2898010 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AA953_RS14400 2892508..2892723 - 216 WP_000839590.1 phage lysis protein EssD -
AA953_RS14405 2893477..2893692 - 216 WP_000066484.1 cold shock-like protein CspB -
AA953_RS14410 2893993..2894205 + 213 WP_000087756.1 cold shock-like protein CspF -
AA953_RS26540 2894260..2894349 + 90 WP_120795389.1 hypothetical protein -
AA953_RS14415 2894627..2895379 - 753 WP_001047135.1 antitermination protein -
AA953_RS14420 2895393..2896442 - 1050 WP_001265198.1 DUF968 domain-containing protein -
AA953_RS14425 2896444..2896722 - 279 WP_012304870.1 hypothetical protein -
AA953_RS14430 2896789..2897040 - 252 WP_000980994.1 hypothetical protein -
AA953_RS14435 2897257..2897412 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AA953_RS14440 2897484..2897771 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AA953_RS14445 2897771..2898010 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AA953_RS24955 2898035..2898340 + 306 WP_001326990.1 hypothetical protein -
AA953_RS14450 2898543..2898875 + 333 WP_001301033.1 protein FlxA -
AA953_RS23740 2899312..2899461 - 150 WP_011443592.1 hypothetical protein -
AA953_RS14455 2899496..2899774 - 279 Protein_2735 hypothetical protein -
AA953_RS14460 2899758..2899988 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
AA953_RS14465 2900072..2900479 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
AA953_RS14470 2900646..2900801 + 156 WP_000379575.1 DUF1391 family protein -
AA953_RS14475 2900961..2901179 + 219 WP_001171942.1 hypothetical protein -
AA953_RS14485 2901747..2901935 + 189 WP_000854559.1 cell division inhibition protein DicB -
AA953_RS14490 2901932..2902123 + 192 WP_001083297.1 lysis protein YdfD -
AA953_RS14495 2902216..2902983 + 768 Protein_2742 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2885760..2906182 20422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T54325 WP_000323025.1 NZ_CP011495:c2897771-2897484 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T54325 NZ_CP011495:c2897771-2897484 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT54325 WP_000534858.1 NZ_CP011495:c2898010-2897771 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT54325 NZ_CP011495:c2898010-2897771 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References