Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1627036..1627562 Replicon chromosome
Accession NZ_CP011342
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag U068_RS08110 Protein ID WP_000323025.1
Coordinates 1627036..1627323 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag U068_RS08115 Protein ID WP_000534858.1
Coordinates 1627323..1627562 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U068_RS08070 1622060..1622275 - 216 WP_000839590.1 phage lysis protein EssD -
U068_RS25800 1622495..1622665 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
U068_RS08075 1623029..1623244 - 216 WP_000066484.1 cold shock-like protein CspB -
U068_RS08080 1623545..1623757 + 213 WP_000087756.1 cold shock-like protein CspF -
U068_RS25355 1623812..1623901 + 90 WP_120795389.1 hypothetical protein -
U068_RS08085 1624179..1624931 - 753 WP_001047135.1 antitermination protein -
U068_RS08090 1624945..1625994 - 1050 WP_001265198.1 DUF968 domain-containing protein -
U068_RS08095 1625996..1626274 - 279 WP_012304870.1 hypothetical protein -
U068_RS08100 1626341..1626592 - 252 WP_000980994.1 hypothetical protein -
U068_RS08105 1626809..1626964 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
U068_RS08110 1627036..1627323 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
U068_RS08115 1627323..1627562 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
U068_RS23555 1627587..1627892 + 306 WP_001326990.1 hypothetical protein -
U068_RS08120 1628095..1628427 + 333 WP_001301033.1 protein FlxA -
U068_RS08125 1628864..1629013 - 150 WP_011443592.1 hypothetical protein -
U068_RS08130 1629048..1629326 - 279 Protein_1557 hypothetical protein -
U068_RS08135 1629310..1629540 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
U068_RS08140 1629624..1630031 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
U068_RS08145 1630198..1630353 + 156 WP_000379575.1 DUF1391 family protein -
U068_RS08150 1630513..1630731 + 219 WP_001171942.1 hypothetical protein -
U068_RS08160 1631299..1631487 + 189 WP_000854559.1 cell division inhibition protein DicB -
U068_RS08165 1631484..1631675 + 192 WP_001083297.1 lysis protein YdfD -
U068_RS23570 1631768..1632535 + 768 Protein_1564 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1612692..1634398 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T54060 WP_000323025.1 NZ_CP011342:c1627323-1627036 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T54060 NZ_CP011342:c1627323-1627036 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT54060 WP_000534858.1 NZ_CP011342:c1627562-1627323 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT54060 NZ_CP011342:c1627562-1627323 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References