54060

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1627036..1627562 Replicon chromosome
Accession NZ_CP011342
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag U068_RS08110 Protein ID WP_000323025.1
Coordinates 1627036..1627323 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag U068_RS08115 Protein ID WP_000534858.1
Coordinates 1627323..1627562 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U068_RS08070 1622060..1622275 - 216 WP_000839590.1 phage lysis protein EssD -
U068_RS25800 1622495..1622665 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
U068_RS08075 1623029..1623244 - 216 WP_000066484.1 cold shock-like protein CspB -
U068_RS08080 1623545..1623757 + 213 WP_000087756.1 cold shock-like protein CspF -
U068_RS25355 1623812..1623901 + 90 WP_120795389.1 hypothetical protein -
U068_RS08085 1624179..1624931 - 753 WP_001047135.1 antitermination protein -
U068_RS08090 1624945..1625994 - 1050 WP_001265198.1 DUF968 domain-containing protein -
U068_RS08095 1625996..1626274 - 279 WP_012304870.1 hypothetical protein -
U068_RS08100 1626341..1626592 - 252 WP_000980994.1 hypothetical protein -
U068_RS08105 1626809..1626964 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
U068_RS08110 1627036..1627323 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
U068_RS08115 1627323..1627562 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
U068_RS23555 1627587..1627892 + 306 WP_001326990.1 hypothetical protein -
U068_RS08120 1628095..1628427 + 333 WP_001301033.1 protein FlxA -
U068_RS08125 1628864..1629013 - 150 WP_011443592.1 hypothetical protein -
U068_RS08130 1629048..1629326 - 279 Protein_1557 hypothetical protein -
U068_RS08135 1629310..1629540 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
U068_RS08140 1629624..1630031 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
U068_RS08145 1630198..1630353 + 156 WP_000379575.1 DUF1391 family protein -
U068_RS08150 1630513..1630731 + 219 WP_001171942.1 hypothetical protein -
U068_RS08160 1631299..1631487 + 189 WP_000854559.1 cell division inhibition protein DicB -
U068_RS08165 1631484..1631675 + 192 WP_001083297.1 lysis protein YdfD -
U068_RS23570 1631768..1632535 + 768 Protein_1564 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1612692..1634398 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T54060 WP_000323025.1 NZ_CP011342:c1627323-1627036 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T54060 NZ_CP011342:c1627323-1627036 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT54060 WP_000534858.1 NZ_CP011342:c1627562-1627323 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT54060 NZ_CP011342:c1627562-1627323 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References