Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1637833..1638359 Replicon chromosome
Accession NZ_CP011324
Organism Escherichia coli strain SQ2203

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CS35_RS08155 Protein ID WP_000323025.1
Coordinates 1637833..1638120 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CS35_RS08160 Protein ID WP_000534858.1
Coordinates 1638120..1638359 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS35_RS08115 1632857..1633072 - 216 WP_000839590.1 phage lysis protein EssD -
CS35_RS08120 1633826..1634041 - 216 WP_000066484.1 cold shock-like protein CspB -
CS35_RS08125 1634342..1634554 + 213 WP_000087756.1 cold shock-like protein CspF -
CS35_RS25445 1634609..1634698 + 90 WP_120795389.1 hypothetical protein -
CS35_RS08130 1634976..1635728 - 753 WP_001047135.1 antitermination protein -
CS35_RS08135 1635742..1636791 - 1050 WP_001393597.1 DUF968 domain-containing protein -
CS35_RS08140 1636793..1637071 - 279 WP_012304870.1 hypothetical protein -
CS35_RS08145 1637138..1637389 - 252 WP_000980994.1 hypothetical protein -
CS35_RS08150 1637606..1637761 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CS35_RS08155 1637833..1638120 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CS35_RS08160 1638120..1638359 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CS35_RS23650 1638384..1638689 + 306 WP_001326990.1 hypothetical protein -
CS35_RS08165 1638892..1639224 + 333 WP_001301033.1 protein FlxA -
CS35_RS22915 1639661..1639810 - 150 WP_011443592.1 hypothetical protein -
CS35_RS08170 1639845..1640123 - 279 Protein_1572 hypothetical protein -
CS35_RS08175 1640107..1640337 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
CS35_RS08180 1640421..1640828 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
CS35_RS08185 1640995..1641150 + 156 WP_000379575.1 DUF1391 family protein -
CS35_RS08190 1641310..1641528 + 219 WP_001171942.1 hypothetical protein -
CS35_RS08200 1642096..1642284 + 189 WP_000854559.1 cell division inhibition protein DicB -
CS35_RS08205 1642281..1642472 + 192 WP_001083297.1 lysis protein YdfD -
CS35_RS23665 1642565..1643332 + 768 Protein_1579 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1626109..1645195 19086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53973 WP_000323025.1 NZ_CP011324:c1638120-1637833 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53973 NZ_CP011324:c1638120-1637833 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53973 WP_000534858.1 NZ_CP011324:c1638359-1638120 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53973 NZ_CP011324:c1638359-1638120 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References