Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1637832..1638358 Replicon chromosome
Accession NZ_CP011322
Organism Escherichia coli strain SQ110

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SG46_RS07995 Protein ID WP_000323025.1
Coordinates 1637832..1638119 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SG46_RS08000 Protein ID WP_000534858.1
Coordinates 1638119..1638358 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SG46_RS07950 1632856..1633071 - 216 WP_000839590.1 phage lysis protein EssD -
SG46_RS07955 1633825..1634040 - 216 WP_000066484.1 cold shock-like protein CspB -
SG46_RS07960 1634341..1634553 + 213 WP_000087756.1 cold shock-like protein CspF -
SG46_RS07965 1634608..1634697 + 90 WP_120795389.1 hypothetical protein -
SG46_RS07970 1634975..1635727 - 753 WP_001047135.1 antitermination protein -
SG46_RS07975 1635741..1636790 - 1050 WP_001393597.1 DUF968 domain-containing protein -
SG46_RS07980 1636792..1637070 - 279 WP_012304870.1 hypothetical protein -
SG46_RS07985 1637137..1637388 - 252 WP_000980994.1 hypothetical protein -
SG46_RS07990 1637605..1637760 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SG46_RS07995 1637832..1638119 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SG46_RS08000 1638119..1638358 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SG46_RS08005 1638383..1638688 + 306 WP_001326990.1 hypothetical protein -
SG46_RS08010 1638891..1639223 + 333 WP_001301033.1 protein FlxA -
SG46_RS08015 1639660..1639809 - 150 WP_011443592.1 hypothetical protein -
SG46_RS08020 1639844..1640122 - 279 Protein_1572 hypothetical protein -
SG46_RS08025 1640106..1640336 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SG46_RS08030 1640420..1640827 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SG46_RS08035 1640994..1641149 + 156 WP_000379575.1 DUF1391 family protein -
SG46_RS08040 1641309..1641527 + 219 WP_001171942.1 hypothetical protein -
SG46_RS08045 1642095..1642283 + 189 WP_000854559.1 cell division inhibition protein DicB -
SG46_RS08050 1642280..1642471 + 192 WP_001083297.1 lysis protein YdfD -
SG46_RS08055 1642564..1643331 + 768 Protein_1579 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1626108..1645194 19086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53930 WP_000323025.1 NZ_CP011322:c1638119-1637832 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53930 NZ_CP011322:c1638119-1637832 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53930 WP_000534858.1 NZ_CP011322:c1638358-1638119 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53930 NZ_CP011322:c1638358-1638119 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References