Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1643371..1643897 Replicon chromosome
Accession NZ_CP011320
Organism Escherichia coli strain SQ37

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SG48_RS08185 Protein ID WP_000323025.1
Coordinates 1643371..1643658 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SG48_RS08190 Protein ID WP_000534858.1
Coordinates 1643658..1643897 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SG48_RS08145 1638395..1638610 - 216 WP_000839590.1 phage lysis protein EssD -
SG48_RS08150 1639364..1639579 - 216 WP_000066484.1 cold shock-like protein CspB -
SG48_RS08155 1639880..1640092 + 213 WP_000087756.1 cold shock-like protein CspF -
SG48_RS25570 1640147..1640236 + 90 WP_120795389.1 hypothetical protein -
SG48_RS08160 1640514..1641266 - 753 WP_001047135.1 antitermination protein -
SG48_RS08165 1641280..1642329 - 1050 WP_001393597.1 DUF968 domain-containing protein -
SG48_RS08170 1642331..1642609 - 279 WP_012304870.1 hypothetical protein -
SG48_RS08175 1642676..1642927 - 252 WP_000980994.1 hypothetical protein -
SG48_RS08180 1643144..1643299 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SG48_RS08185 1643371..1643658 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SG48_RS08190 1643658..1643897 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SG48_RS23790 1643922..1644227 + 306 WP_001326990.1 hypothetical protein -
SG48_RS08195 1644430..1644762 + 333 WP_001301033.1 protein FlxA -
SG48_RS23055 1645199..1645348 - 150 WP_011443592.1 hypothetical protein -
SG48_RS08200 1645383..1645661 - 279 Protein_1572 hypothetical protein -
SG48_RS08205 1645645..1645875 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SG48_RS08210 1645959..1646366 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SG48_RS08215 1646533..1646688 + 156 WP_000379575.1 DUF1391 family protein -
SG48_RS08220 1646848..1647066 + 219 WP_001171942.1 hypothetical protein -
SG48_RS08230 1647634..1647822 + 189 WP_000854559.1 cell division inhibition protein DicB -
SG48_RS08235 1647819..1648010 + 192 WP_001083297.1 lysis protein YdfD -
SG48_RS23805 1648103..1648870 + 768 Protein_1579 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1629027..1650733 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T53844 WP_000323025.1 NZ_CP011320:c1643658-1643371 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T53844 NZ_CP011320:c1643658-1643371 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT53844 WP_000534858.1 NZ_CP011320:c1643897-1643658 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT53844 NZ_CP011320:c1643897-1643658 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References