Detailed information of TA system TA053038

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3320205..3320414
• Replicon: chromosome
• Accession: NZ_CP010888
• Organism: Clostridioides difficile strain 08ACD0030

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: TW87_RS15755
• Protein ID: WP_004454815.1
• Coordinates: 3320256..3320414 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	3320205..3320339 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TW87_RS15740	3315832..3317460	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
TW87_RS15745	3317581..3318576	+	996	WP_003431031.1	asparaginase	-
TW87_RS15750	3318765..3319148	-	384	Protein_3003	BlaI/MecI/CopY family transcriptional regulator	-
-	3320205..3320339	+	135	NuclAT_2	-	Antitoxin
TW87_RS15755	3320256..3320414	-	159	WP_004454815.1	hypothetical protein	Toxin
TW87_RS15760	3320773..3322143	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
TW87_RS19470	3322426..3322524	+	99	Protein_3006	Arm DNA-binding domain-containing protein	-
TW87_RS19645	3322543..3322964	+	422	Protein_3007	hypothetical protein	-
TW87_RS19480	3323195..3323467	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
TW87_RS15775	3323785..3324453	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T53038 WP_004454815.1 NZ_CP010888:c3320414-3320256 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T53038 NZ_CP010888:c3320414-3320256 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATATAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT53038 NZ_CP010888:3320205-3320339 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin