Detailed information of TA system TA053029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1685781..1685996
• Replicon: chromosome
• Accession: NZ_CP010888
• Organism: Clostridioides difficile strain 08ACD0030

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: TW87_RS08055
• Protein ID: WP_011861731.1
• Coordinates: 1685781..1685924 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	1685846..1685996 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TW87_RS08030	1681438..1681800	+	363	WP_015984864.1	hypothetical protein	-
TW87_RS08035	1681966..1682484	+	519	WP_009898407.1	hypothetical protein	-
TW87_RS08040	1682548..1684134	-	1587	WP_015984865.1	hypothetical protein	-
TW87_RS08045	1684110..1684631	-	522	WP_009898403.1	ImmA/IrrE family metallo-endopeptidase	-
TW87_RS08050	1685333..1685521	-	189	WP_009898401.1	hypothetical protein	-
TW87_RS08055	1685781..1685924	+	144	WP_011861731.1	hypothetical protein	Toxin
-	1685846..1685996	-	151	NuclAT_1	-	Antitoxin
-	1685850..1685996	-	147	NuclAT_0	-	-
TW87_RS08060	1686431..1686808	+	378	WP_021391346.1	BlaI/MecI/CopY family transcriptional regulator	-
TW87_RS08065	1686918..1687301	+	384	WP_015984867.1	BlaI/MecI/CopY family transcriptional regulator	-
TW87_RS08070	1687486..1688070	+	585	Protein_1481	GntR family transcriptional regulator	-
TW87_RS08075	1688096..1688509	+	414	WP_009896035.1	PTS sugar transporter subunit IIA	-
TW87_RS08080	1688513..1689256	+	744	WP_003437997.1	membrane complex biogenesis protein, BtpA family	-
TW87_RS08085	1689256..1689726	+	471	WP_003428544.1	PTS sugar transporter subunit IIB	-
TW87_RS08090	1689743..1690501	+	759	WP_009888882.1	PTS sugar transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1627875..1708027	80152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T53029 WP_011861731.1 NZ_CP010888:1685781-1685924 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T53029 NZ_CP010888:1685781-1685924 [Clostridioides difficile]
ATGAGCGAATTTTTACTAGGAGTGTTAGCTAGTTTAACAGCTAGCTTTATTACATATATTATTTCCAGAAAAGTAAAAAG
CCACTCTGGCAGGAGTGACTTTGAGCTTGATGTAAAAATCAAGTTTAATAAAAAACGACATTAA

Antitoxin

Nucleotide

• Length: 151 bp

>AT53029 NZ_CP010888:c1685996-1685846 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTTTCTG

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin