Detailed information of TA system TA053027

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1660568..1660788
• Replicon: chromosome
• Accession: NZ_CP010888
• Organism: Clostridioides difficile strain 08ACD0030

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: A0A069AVC0
• Locus tag: TW87_RS19995
• Protein ID: WP_009901465.1
• Coordinates: 1660568..1660729 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1660649..1660788 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TW87_RS07865	1655680..1655913	+	234	WP_003429842.1	hypothetical protein	-
TW87_RS07870	1655923..1656324	+	402	WP_015984842.1	hypothetical protein	-
TW87_RS07875	1656318..1656665	+	348	WP_015984843.1	hypothetical protein	-
TW87_RS07880	1656665..1657090	+	426	WP_009896626.1	HK97 gp10 family phage protein	-
TW87_RS07885	1657083..1657520	+	438	WP_009901457.1	hypothetical protein	-
TW87_RS07890	1657513..1657689	+	177	WP_021368348.1	hypothetical protein	-
TW87_RS07895	1657690..1659000	+	1311	WP_015984844.1	phage tail sheath family protein	-
TW87_RS07900	1659022..1659492	+	471	WP_015984845.1	phage tail tube protein	-
TW87_RS07905	1659545..1659802	+	258	WP_015984846.1	hypothetical protein	-
TW87_RS07910	1659807..1660247	+	441	WP_015984847.1	hypothetical protein	-
TW87_RS19995	1660568..1660729	+	162	WP_009901465.1	hypothetical protein	Toxin
-	1660649..1660788	-	140	NuclAT_7	-	Antitoxin
-	1660649..1660788	-	140	NuclAT_8	-	Antitoxin
-	1661133..1661238	-	106	NuclAT_4	-	-
-	1661133..1661238	-	106	NuclAT_5	-	-
TW87_RS07920	1661992..1662180	+	189	WP_003429858.1	hypothetical protein	-
TW87_RS07925	1662299..1663165	+	867	WP_021378510.1	hypothetical protein	-
TW87_RS20000	1663218..1663373	+	156	WP_021378513.1	hypothetical protein	-
TW87_RS07930	1663792..1664004	-	213	WP_016728883.1	helix-turn-helix transcriptional regulator	-
TW87_RS19690	1664279..1664623	+	345	Protein_1452	ORF6N domain-containing protein	-
TW87_RS19695	1664705..1665058	+	354	WP_095206559.1	hypothetical protein	-
TW87_RS20005	1665091..1665243	+	153	WP_021378619.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1627875..1708027	80152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6034.28 Da
• Isoelectric Point: 11.0253

>T53027 WP_009901465.1 NZ_CP010888:1660568-1660729 [Clostridioides difficile]
MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELGFKIKFRKFK

Nucleotide

• Length: 162 bp

>T53027 NZ_CP010888:1660568-1660729 [Clostridioides difficile]
ATGGACAATTTTTTACTTAGTATATTAGCTAGCTTGATAGCTAGTTTAATTGGATATATCGTTTGTAGATGTATCAAAAA
CGTAAAAAGCCACTCTACTCGTGGCAAGAGTAAAAGTGGCTGGGAACTTGGTTTTAAAATAAAGTTCCGCAAATTTAAAT
AA

Antitoxin

Nucleotide

• Length: 140 bp

>AT53027 NZ_CP010888:c1660788-1660649 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGCGGTAGAGTGAAGTTCATAATTTTAAAAAATATAAATTATTTAAATTTGCGGAACTT
TATTTTAAAACCAAGTTCCCAGCCACTTTTACTCTTGCCACGAGTAGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVC0

Antitoxin