Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2441714..2442240 Replicon chromosome
Accession NZ_CP010445
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SF31_RS12115 Protein ID WP_000323025.1
Coordinates 2441714..2442001 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SF31_RS12120 Protein ID WP_000534858.1
Coordinates 2442001..2442240 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF31_RS12075 2436738..2436953 - 216 WP_000839590.1 phage lysis protein EssD -
SF31_RS12080 2437707..2437922 - 216 WP_000066484.1 cold shock-like protein CspB -
SF31_RS12085 2438223..2438435 + 213 WP_000087756.1 cold shock-like protein CspF -
SF31_RS26010 2438490..2438579 + 90 WP_120795389.1 hypothetical protein -
SF31_RS12090 2438857..2439609 - 753 WP_001047135.1 antitermination protein -
SF31_RS12095 2439623..2440672 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SF31_RS12100 2440674..2440952 - 279 WP_012304870.1 hypothetical protein -
SF31_RS12105 2441019..2441270 - 252 WP_000980994.1 hypothetical protein -
SF31_RS12110 2441487..2441642 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SF31_RS12115 2441714..2442001 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SF31_RS12120 2442001..2442240 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SF31_RS24420 2442265..2442570 + 306 WP_001326990.1 hypothetical protein -
SF31_RS12125 2442773..2443105 + 333 WP_001301033.1 protein FlxA -
SF31_RS23345 2443542..2443691 - 150 WP_011443592.1 hypothetical protein -
SF31_RS12130 2443726..2444004 - 279 Protein_2285 hypothetical protein -
SF31_RS12135 2443988..2444218 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SF31_RS12140 2444302..2444709 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SF31_RS12145 2444876..2445031 + 156 WP_000379575.1 DUF1391 family protein -
SF31_RS12150 2445191..2445409 + 219 WP_001171942.1 hypothetical protein -
SF31_RS12160 2445977..2446165 + 189 WP_000854559.1 cell division inhibition protein DicB -
SF31_RS12165 2446162..2446353 + 192 WP_001083297.1 lysis protein YdfD -
SF31_RS24435 2446446..2447213 + 768 Protein_2292 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2427370..2449076 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52714 WP_000323025.1 NZ_CP010445:c2442001-2441714 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52714 NZ_CP010445:c2442001-2441714 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52714 WP_000534858.1 NZ_CP010445:c2442240-2442001 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52714 NZ_CP010445:c2442240-2442001 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References