52714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2441714..2442240 Replicon chromosome
Accession NZ_CP010445
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SF31_RS12115 Protein ID WP_000323025.1
Coordinates 2441714..2442001 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SF31_RS12120 Protein ID WP_000534858.1
Coordinates 2442001..2442240 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF31_RS12075 2436738..2436953 - 216 WP_000839590.1 phage lysis protein EssD -
SF31_RS12080 2437707..2437922 - 216 WP_000066484.1 cold shock-like protein CspB -
SF31_RS12085 2438223..2438435 + 213 WP_000087756.1 cold shock-like protein CspF -
SF31_RS26010 2438490..2438579 + 90 WP_120795389.1 hypothetical protein -
SF31_RS12090 2438857..2439609 - 753 WP_001047135.1 antitermination protein -
SF31_RS12095 2439623..2440672 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SF31_RS12100 2440674..2440952 - 279 WP_012304870.1 hypothetical protein -
SF31_RS12105 2441019..2441270 - 252 WP_000980994.1 hypothetical protein -
SF31_RS12110 2441487..2441642 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SF31_RS12115 2441714..2442001 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SF31_RS12120 2442001..2442240 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SF31_RS24420 2442265..2442570 + 306 WP_001326990.1 hypothetical protein -
SF31_RS12125 2442773..2443105 + 333 WP_001301033.1 protein FlxA -
SF31_RS23345 2443542..2443691 - 150 WP_011443592.1 hypothetical protein -
SF31_RS12130 2443726..2444004 - 279 Protein_2285 hypothetical protein -
SF31_RS12135 2443988..2444218 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SF31_RS12140 2444302..2444709 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SF31_RS12145 2444876..2445031 + 156 WP_000379575.1 DUF1391 family protein -
SF31_RS12150 2445191..2445409 + 219 WP_001171942.1 hypothetical protein -
SF31_RS12160 2445977..2446165 + 189 WP_000854559.1 cell division inhibition protein DicB -
SF31_RS12165 2446162..2446353 + 192 WP_001083297.1 lysis protein YdfD -
SF31_RS24435 2446446..2447213 + 768 Protein_2292 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2427370..2449076 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52714 WP_000323025.1 NZ_CP010445:c2442001-2441714 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52714 NZ_CP010445:c2442001-2441714 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52714 WP_000534858.1 NZ_CP010445:c2442240-2442001 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52714 NZ_CP010445:c2442240-2442001 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References