Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2390913..2391439 Replicon chromosome
Accession NZ_CP010444
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH07_RS11800 Protein ID WP_000323025.1
Coordinates 2390913..2391200 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH07_RS11805 Protein ID WP_000534858.1
Coordinates 2391200..2391439 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH07_RS11760 2385937..2386152 - 216 WP_000839590.1 phage lysis protein EssD -
SH07_RS11765 2386906..2387121 - 216 WP_000066484.1 cold shock-like protein CspB -
SH07_RS11770 2387422..2387634 + 213 WP_000087756.1 cold shock-like protein CspF -
SH07_RS25580 2387689..2387778 + 90 WP_120795389.1 hypothetical protein -
SH07_RS11775 2388056..2388808 - 753 WP_001047135.1 antitermination protein -
SH07_RS11780 2388822..2389871 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH07_RS11785 2389873..2390151 - 279 WP_012304870.1 hypothetical protein -
SH07_RS11790 2390218..2390469 - 252 WP_000980994.1 hypothetical protein -
SH07_RS11795 2390686..2390841 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH07_RS11800 2390913..2391200 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH07_RS11805 2391200..2391439 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH07_RS24005 2391464..2391769 + 306 WP_001326990.1 hypothetical protein -
SH07_RS11810 2391972..2392304 + 333 WP_001301033.1 protein FlxA -
SH07_RS22975 2392741..2392890 - 150 WP_011443592.1 hypothetical protein -
SH07_RS11815 2392925..2393203 - 279 Protein_2227 hypothetical protein -
SH07_RS11820 2393187..2393417 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH07_RS11825 2393501..2393908 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH07_RS11830 2394075..2394230 + 156 WP_000379575.1 DUF1391 family protein -
SH07_RS11835 2394390..2394608 + 219 WP_001171942.1 hypothetical protein -
SH07_RS11845 2395176..2395364 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH07_RS11850 2395361..2395552 + 192 WP_038432577.1 lysis protein YdfD -
SH07_RS24020 2395645..2396412 + 768 Protein_2234 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2376569..2399482 22913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52672 WP_000323025.1 NZ_CP010444:c2391200-2390913 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52672 NZ_CP010444:c2391200-2390913 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52672 WP_000534858.1 NZ_CP010444:c2391439-2391200 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52672 NZ_CP010444:c2391439-2391200 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References