Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2388143..2388669 Replicon chromosome
Accession NZ_CP010443
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH04_RS11795 Protein ID WP_000323025.1
Coordinates 2388143..2388430 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH04_RS11800 Protein ID WP_000534858.1
Coordinates 2388430..2388669 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH04_RS11755 2383167..2383382 - 216 WP_000839590.1 phage lysis protein EssD -
SH04_RS11760 2384136..2384351 - 216 WP_000066484.1 cold shock-like protein CspB -
SH04_RS11765 2384652..2384864 + 213 WP_000087756.1 cold shock-like protein CspF -
SH04_RS25570 2384919..2385008 + 90 WP_120795389.1 hypothetical protein -
SH04_RS11770 2385286..2386038 - 753 WP_001047135.1 antitermination protein -
SH04_RS11775 2386052..2387101 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH04_RS11780 2387103..2387381 - 279 WP_012304870.1 hypothetical protein -
SH04_RS11785 2387448..2387699 - 252 WP_000980994.1 hypothetical protein -
SH04_RS11790 2387916..2388071 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH04_RS11795 2388143..2388430 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH04_RS11800 2388430..2388669 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH04_RS24010 2388694..2388999 + 306 WP_001326990.1 hypothetical protein -
SH04_RS11805 2389202..2389534 + 333 WP_001301033.1 protein FlxA -
SH04_RS22970 2389971..2390120 - 150 WP_011443592.1 hypothetical protein -
SH04_RS11810 2390155..2390433 - 279 Protein_2225 hypothetical protein -
SH04_RS11815 2390417..2390647 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH04_RS11820 2390731..2391138 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH04_RS11825 2391305..2391460 + 156 WP_000379575.1 DUF1391 family protein -
SH04_RS11830 2391620..2391838 + 219 WP_001171942.1 hypothetical protein -
SH04_RS11840 2392406..2392594 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH04_RS11845 2392591..2392782 + 192 WP_038432577.1 lysis protein YdfD -
SH04_RS24025 2392875..2393642 + 768 Protein_2232 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2357601..2412389 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52631 WP_000323025.1 NZ_CP010443:c2388430-2388143 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52631 NZ_CP010443:c2388430-2388143 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52631 WP_000534858.1 NZ_CP010443:c2388669-2388430 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52631 NZ_CP010443:c2388669-2388430 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References