Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2441715..2442241 Replicon chromosome
Accession NZ_CP010442
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH06_RS12115 Protein ID WP_000323025.1
Coordinates 2441715..2442002 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH06_RS12120 Protein ID WP_000534858.1
Coordinates 2442002..2442241 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH06_RS12075 2436739..2436954 - 216 WP_000839590.1 phage lysis protein EssD -
SH06_RS12080 2437708..2437923 - 216 WP_000066484.1 cold shock-like protein CspB -
SH06_RS12085 2438224..2438436 + 213 WP_000087756.1 cold shock-like protein CspF -
SH06_RS25955 2438491..2438580 + 90 WP_120795389.1 hypothetical protein -
SH06_RS12090 2438858..2439610 - 753 WP_001047135.1 antitermination protein -
SH06_RS12095 2439624..2440673 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH06_RS12100 2440675..2440953 - 279 WP_012304870.1 hypothetical protein -
SH06_RS12105 2441020..2441271 - 252 WP_000980994.1 hypothetical protein -
SH06_RS12110 2441488..2441643 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH06_RS12115 2441715..2442002 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH06_RS12120 2442002..2442241 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH06_RS24355 2442266..2442571 + 306 WP_001326990.1 hypothetical protein -
SH06_RS12125 2442774..2443106 + 333 WP_001301033.1 protein FlxA -
SH06_RS23285 2443543..2443692 - 150 WP_011443592.1 hypothetical protein -
SH06_RS12130 2443727..2444005 - 279 Protein_2285 hypothetical protein -
SH06_RS12135 2443989..2444219 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH06_RS12140 2444303..2444710 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH06_RS12145 2444877..2445032 + 156 WP_000379575.1 DUF1391 family protein -
SH06_RS12150 2445192..2445410 + 219 WP_001171942.1 hypothetical protein -
SH06_RS12160 2445978..2446166 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH06_RS12165 2446163..2446354 + 192 WP_038432577.1 lysis protein YdfD -
SH06_RS24370 2446447..2447214 + 768 Protein_2292 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2427371..2449077 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52590 WP_000323025.1 NZ_CP010442:c2442002-2441715 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52590 NZ_CP010442:c2442002-2441715 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52590 WP_000534858.1 NZ_CP010442:c2442241-2442002 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52590 NZ_CP010442:c2442241-2442002 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References