52590

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2441715..2442241 Replicon chromosome
Accession NZ_CP010442
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH06_RS12115 Protein ID WP_000323025.1
Coordinates 2441715..2442002 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH06_RS12120 Protein ID WP_000534858.1
Coordinates 2442002..2442241 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH06_RS12075 2436739..2436954 - 216 WP_000839590.1 phage lysis protein EssD -
SH06_RS12080 2437708..2437923 - 216 WP_000066484.1 cold shock-like protein CspB -
SH06_RS12085 2438224..2438436 + 213 WP_000087756.1 cold shock-like protein CspF -
SH06_RS25955 2438491..2438580 + 90 WP_120795389.1 hypothetical protein -
SH06_RS12090 2438858..2439610 - 753 WP_001047135.1 antitermination protein -
SH06_RS12095 2439624..2440673 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH06_RS12100 2440675..2440953 - 279 WP_012304870.1 hypothetical protein -
SH06_RS12105 2441020..2441271 - 252 WP_000980994.1 hypothetical protein -
SH06_RS12110 2441488..2441643 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH06_RS12115 2441715..2442002 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH06_RS12120 2442002..2442241 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH06_RS24355 2442266..2442571 + 306 WP_001326990.1 hypothetical protein -
SH06_RS12125 2442774..2443106 + 333 WP_001301033.1 protein FlxA -
SH06_RS23285 2443543..2443692 - 150 WP_011443592.1 hypothetical protein -
SH06_RS12130 2443727..2444005 - 279 Protein_2285 hypothetical protein -
SH06_RS12135 2443989..2444219 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH06_RS12140 2444303..2444710 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH06_RS12145 2444877..2445032 + 156 WP_000379575.1 DUF1391 family protein -
SH06_RS12150 2445192..2445410 + 219 WP_001171942.1 hypothetical protein -
SH06_RS12160 2445978..2446166 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH06_RS12165 2446163..2446354 + 192 WP_038432577.1 lysis protein YdfD -
SH06_RS24370 2446447..2447214 + 768 Protein_2292 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2427371..2449077 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52590 WP_000323025.1 NZ_CP010442:c2442002-2441715 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52590 NZ_CP010442:c2442002-2441715 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52590 WP_000534858.1 NZ_CP010442:c2442241-2442002 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52590 NZ_CP010442:c2442241-2442002 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References