52507

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2402458..2402984 Replicon chromosome
Accession NZ_CP010440
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH08_RS11900 Protein ID WP_000323025.1
Coordinates 2402458..2402745 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH08_RS11905 Protein ID WP_000534858.1
Coordinates 2402745..2402984 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH08_RS11860 2397482..2397697 - 216 WP_000839590.1 phage lysis protein EssD -
SH08_RS11865 2398451..2398666 - 216 WP_000066484.1 cold shock-like protein CspB -
SH08_RS11870 2398967..2399179 + 213 WP_000087756.1 cold shock-like protein CspF -
SH08_RS25750 2399234..2399323 + 90 WP_120795389.1 hypothetical protein -
SH08_RS11875 2399601..2400353 - 753 WP_001047135.1 antitermination protein -
SH08_RS11880 2400367..2401416 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH08_RS11885 2401418..2401696 - 279 WP_012304870.1 hypothetical protein -
SH08_RS11890 2401763..2402014 - 252 WP_000980994.1 hypothetical protein -
SH08_RS11895 2402231..2402386 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH08_RS11900 2402458..2402745 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH08_RS11905 2402745..2402984 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH08_RS24150 2403009..2403314 + 306 WP_001326990.1 hypothetical protein -
SH08_RS11910 2403517..2403849 + 333 WP_001301033.1 protein FlxA -
SH08_RS23075 2404286..2404435 - 150 WP_011443592.1 hypothetical protein -
SH08_RS11915 2404470..2404748 - 279 Protein_2251 hypothetical protein -
SH08_RS11920 2404732..2404962 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH08_RS11925 2405046..2405453 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH08_RS11930 2405620..2405775 + 156 WP_000379575.1 DUF1391 family protein -
SH08_RS11935 2405935..2406153 + 219 WP_001171942.1 hypothetical protein -
SH08_RS11945 2406721..2406909 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH08_RS11950 2406906..2407097 + 192 WP_038432577.1 lysis protein YdfD -
SH08_RS24165 2407190..2407957 + 768 Protein_2258 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2388114..2409820 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52507 WP_000323025.1 NZ_CP010440:c2402745-2402458 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52507 NZ_CP010440:c2402745-2402458 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52507 WP_000534858.1 NZ_CP010440:c2402984-2402745 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52507 NZ_CP010440:c2402984-2402745 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References