Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2402458..2402984 Replicon chromosome
Accession NZ_CP010440
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH08_RS11900 Protein ID WP_000323025.1
Coordinates 2402458..2402745 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH08_RS11905 Protein ID WP_000534858.1
Coordinates 2402745..2402984 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH08_RS11860 2397482..2397697 - 216 WP_000839590.1 phage lysis protein EssD -
SH08_RS11865 2398451..2398666 - 216 WP_000066484.1 cold shock-like protein CspB -
SH08_RS11870 2398967..2399179 + 213 WP_000087756.1 cold shock-like protein CspF -
SH08_RS25750 2399234..2399323 + 90 WP_120795389.1 hypothetical protein -
SH08_RS11875 2399601..2400353 - 753 WP_001047135.1 antitermination protein -
SH08_RS11880 2400367..2401416 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH08_RS11885 2401418..2401696 - 279 WP_012304870.1 hypothetical protein -
SH08_RS11890 2401763..2402014 - 252 WP_000980994.1 hypothetical protein -
SH08_RS11895 2402231..2402386 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH08_RS11900 2402458..2402745 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH08_RS11905 2402745..2402984 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH08_RS24150 2403009..2403314 + 306 WP_001326990.1 hypothetical protein -
SH08_RS11910 2403517..2403849 + 333 WP_001301033.1 protein FlxA -
SH08_RS23075 2404286..2404435 - 150 WP_011443592.1 hypothetical protein -
SH08_RS11915 2404470..2404748 - 279 Protein_2251 hypothetical protein -
SH08_RS11920 2404732..2404962 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH08_RS11925 2405046..2405453 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH08_RS11930 2405620..2405775 + 156 WP_000379575.1 DUF1391 family protein -
SH08_RS11935 2405935..2406153 + 219 WP_001171942.1 hypothetical protein -
SH08_RS11945 2406721..2406909 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH08_RS11950 2406906..2407097 + 192 WP_038432577.1 lysis protein YdfD -
SH08_RS24165 2407190..2407957 + 768 Protein_2258 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2388114..2409820 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52507 WP_000323025.1 NZ_CP010440:c2402745-2402458 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52507 NZ_CP010440:c2402745-2402458 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52507 WP_000534858.1 NZ_CP010440:c2402984-2402745 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52507 NZ_CP010440:c2402984-2402745 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References