Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2401011..2401537 Replicon chromosome
Accession NZ_CP010438
Organism Escherichia coli K-12 strain K-12 MG1655

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag SH05_RS11905 Protein ID WP_000323025.1
Coordinates 2401011..2401298 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag SH05_RS11910 Protein ID WP_000534858.1
Coordinates 2401298..2401537 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SH05_RS11865 2396035..2396250 - 216 WP_000839590.1 phage lysis protein EssD -
SH05_RS11870 2397004..2397219 - 216 WP_000066484.1 cold shock-like protein CspB -
SH05_RS11875 2397520..2397732 + 213 WP_000087756.1 cold shock-like protein CspF -
SH05_RS25720 2397787..2397876 + 90 WP_120795389.1 hypothetical protein -
SH05_RS11880 2398154..2398906 - 753 WP_001047135.1 antitermination protein -
SH05_RS11885 2398920..2399969 - 1050 WP_001265198.1 DUF968 domain-containing protein -
SH05_RS11890 2399971..2400249 - 279 WP_012304870.1 hypothetical protein -
SH05_RS11895 2400316..2400567 - 252 WP_000980994.1 hypothetical protein -
SH05_RS11900 2400784..2400939 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
SH05_RS11905 2401011..2401298 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
SH05_RS11910 2401298..2401537 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
SH05_RS24150 2401562..2401867 + 306 WP_001326990.1 hypothetical protein -
SH05_RS11915 2402070..2402402 + 333 WP_001301033.1 protein FlxA -
SH05_RS23080 2402839..2402988 - 150 WP_011443592.1 hypothetical protein -
SH05_RS11920 2403023..2403301 - 279 Protein_2250 hypothetical protein -
SH05_RS11925 2403285..2403515 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
SH05_RS11930 2403599..2404006 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
SH05_RS11935 2404173..2404328 + 156 WP_000379575.1 DUF1391 family protein -
SH05_RS11940 2404488..2404706 + 219 WP_001171942.1 hypothetical protein -
SH05_RS11950 2405274..2405462 + 189 WP_000854559.1 cell division inhibition protein DicB -
SH05_RS11955 2405459..2405650 + 192 WP_038432577.1 lysis protein YdfD -
SH05_RS24165 2405743..2406510 + 768 Protein_2257 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2386667..2409580 22913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T52436 WP_000323025.1 NZ_CP010438:c2401298-2401011 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T52436 NZ_CP010438:c2401298-2401011 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT52436 WP_000534858.1 NZ_CP010438:c2401537-2401298 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT52436 NZ_CP010438:c2401537-2401298 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References