Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1627113..1627639 Replicon chromosome
Accession NZ_CP009789
Organism Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ER3413_RS08100 Protein ID WP_000323025.1
Coordinates 1627113..1627400 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ER3413_RS08105 Protein ID WP_000534858.1
Coordinates 1627400..1627639 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER3413_RS08060 1622137..1622352 - 216 WP_000839590.1 phage lysis protein EssD -
ER3413_RS08065 1623106..1623321 - 216 WP_000066484.1 cold shock-like protein CspB -
ER3413_RS08070 1623622..1623834 + 213 WP_000087756.1 cold shock-like protein CspF -
ER3413_RS25310 1623889..1623978 + 90 WP_120795389.1 hypothetical protein -
ER3413_RS08075 1624256..1625008 - 753 WP_001047135.1 antitermination protein -
ER3413_RS08080 1625022..1626071 - 1050 WP_001265198.1 DUF968 domain-containing protein -
ER3413_RS08085 1626073..1626351 - 279 WP_012304870.1 hypothetical protein -
ER3413_RS08090 1626418..1626669 - 252 WP_000980994.1 hypothetical protein -
ER3413_RS08095 1626886..1627041 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ER3413_RS08100 1627113..1627400 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ER3413_RS08105 1627400..1627639 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ER3413_RS23485 1627664..1627969 + 306 WP_001326990.1 hypothetical protein -
ER3413_RS08110 1628172..1628504 + 333 WP_001301033.1 protein FlxA -
ER3413_RS22760 1628941..1629090 - 150 WP_011443592.1 hypothetical protein -
ER3413_RS08115 1629125..1629403 - 279 Protein_1549 hypothetical protein -
ER3413_RS08120 1629387..1629617 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ER3413_RS08125 1629701..1630108 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ER3413_RS08130 1630275..1630430 + 156 WP_000379575.1 DUF1391 family protein -
ER3413_RS08135 1630590..1630808 + 219 WP_038432574.1 hypothetical protein -
ER3413_RS08145 1631376..1631564 + 189 WP_000854559.1 cell division inhibition protein DicB -
ER3413_RS08150 1631561..1631752 + 192 WP_038432577.1 lysis protein YdfD -
ER3413_RS23500 1631845..1632612 + 768 Protein_1556 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1611431..1634475 23044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T49791 WP_000323025.1 NZ_CP009789:c1627400-1627113 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T49791 NZ_CP009789:c1627400-1627113 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT49791 WP_000534858.1 NZ_CP009789:c1627639-1627400 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT49791 NZ_CP009789:c1627639-1627400 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References