Detailed information of TA system TA049791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1627113..1627639
• Replicon: chromosome
• Accession: NZ_CP009789
• Organism: Escherichia coli K-12 strain K-12 ER3413

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: ER3413_RS08100
• Protein ID: WP_000323025.1
• Coordinates: 1627113..1627400 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: ER3413_RS08105
• Protein ID: WP_000534858.1
• Coordinates: 1627400..1627639 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_RS08060	1622137..1622352	-	216	WP_000839590.1	phage lysis protein EssD	-
ER3413_RS08065	1623106..1623321	-	216	WP_000066484.1	cold shock-like protein CspB	-
ER3413_RS08070	1623622..1623834	+	213	WP_000087756.1	cold shock-like protein CspF	-
ER3413_RS25310	1623889..1623978	+	90	WP_120795389.1	hypothetical protein	-
ER3413_RS08075	1624256..1625008	-	753	WP_001047135.1	antitermination protein	-
ER3413_RS08080	1625022..1626071	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
ER3413_RS08085	1626073..1626351	-	279	WP_012304870.1	hypothetical protein	-
ER3413_RS08090	1626418..1626669	-	252	WP_000980994.1	hypothetical protein	-
ER3413_RS08095	1626886..1627041	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
ER3413_RS08100	1627113..1627400	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
ER3413_RS08105	1627400..1627639	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
ER3413_RS23485	1627664..1627969	+	306	WP_001326990.1	hypothetical protein	-
ER3413_RS08110	1628172..1628504	+	333	WP_001301033.1	protein FlxA	-
ER3413_RS22760	1628941..1629090	-	150	WP_011443592.1	hypothetical protein	-
ER3413_RS08115	1629125..1629403	-	279	Protein_1549	hypothetical protein	-
ER3413_RS08120	1629387..1629617	-	231	WP_000920568.1	dicB transcriptional regulator DicC	-
ER3413_RS08125	1629701..1630108	+	408	WP_000448564.1	DNA-binding transcriptional dual regulator DicA	-
ER3413_RS08130	1630275..1630430	+	156	WP_000379575.1	DUF1391 family protein	-
ER3413_RS08135	1630590..1630808	+	219	WP_038432574.1	hypothetical protein	-
ER3413_RS08145	1631376..1631564	+	189	WP_000854559.1	cell division inhibition protein DicB	-
ER3413_RS08150	1631561..1631752	+	192	WP_038432577.1	lysis protein YdfD	-
ER3413_RS23500	1631845..1632612	+	768	Protein_1556	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020026 1611431..1634475 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021438 1632716..1654422 0.002 View NC_017638 Escherichia coli DH1 Prophage TA027404 1615804..1634890 0.004 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Prophage TA068425 1534772..1559021 0.003 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090479 488442..510148 0.002	inside	Prophage	-	-	1611431..1634475	23044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T49791 WP_000323025.1 NZ_CP009789:c1627400-1627113 [Escherichia coli K-12]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T49791 NZ_CP009789:c1627400-1627113 [Escherichia coli K-12]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT49791 WP_000534858.1 NZ_CP009789:c1627639-1627400 [Escherichia coli K-12]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT49791 NZ_CP009789:c1627639-1627400 [Escherichia coli K-12]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8