Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2049409..2049935 Replicon chromosome
Accession NZ_CP009685
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag EO53_RS10155 Protein ID WP_000323025.1
Coordinates 2049648..2049935 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag EO53_RS10150 Protein ID WP_000534858.1
Coordinates 2049409..2049648 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EO53_RS23865 2044436..2045203 - 768 Protein_1916 exonuclease -
EO53_RS10105 2045296..2045487 - 192 WP_001083297.1 lysis protein YdfD -
EO53_RS10110 2045484..2045672 - 189 WP_000854559.1 cell division inhibition protein DicB -
EO53_RS10120 2046240..2046458 - 219 WP_001171942.1 hypothetical protein -
EO53_RS10125 2046618..2046773 - 156 WP_000379575.1 DUF1391 family protein -
EO53_RS10130 2046940..2047347 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
EO53_RS10135 2047431..2047661 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
EO53_RS10140 2047645..2047923 + 279 Protein_1923 hypothetical protein -
EO53_RS23065 2047958..2048107 + 150 WP_011443592.1 hypothetical protein -
EO53_RS10145 2048544..2048876 - 333 WP_001301033.1 protein FlxA -
EO53_RS23880 2049079..2049384 - 306 WP_001326990.1 hypothetical protein -
EO53_RS10150 2049409..2049648 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
EO53_RS10155 2049648..2049935 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
EO53_RS10160 2050007..2050162 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
EO53_RS10165 2050379..2050630 + 252 WP_000980994.1 hypothetical protein -
EO53_RS10170 2050697..2050975 + 279 WP_012304870.1 hypothetical protein -
EO53_RS10175 2050977..2052026 + 1050 WP_001393597.1 DUF968 domain-containing protein -
EO53_RS10180 2052040..2052792 + 753 WP_001047135.1 antitermination protein -
EO53_RS25685 2053070..2053159 - 90 WP_120795389.1 hypothetical protein -
EO53_RS10185 2053214..2053426 - 213 WP_000087756.1 cold shock-like protein CspF -
EO53_RS10190 2053727..2053942 + 216 WP_000066484.1 cold shock-like protein CspB -
EO53_RS10195 2054696..2054911 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2025689..2080477 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T49444 WP_000323025.1 NZ_CP009685:2049648-2049935 [Escherichia coli str. K-12 substr. MG1655]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T49444 NZ_CP009685:2049648-2049935 [Escherichia coli str. K-12 substr. MG1655]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT49444 WP_000534858.1 NZ_CP009685:2049409-2049648 [Escherichia coli str. K-12 substr. MG1655]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT49444 NZ_CP009685:2049409-2049648 [Escherichia coli str. K-12 substr. MG1655]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References