Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1620628..1621154 Replicon chromosome
Accession NZ_CP008801
Organism Escherichia coli KLY

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HQ24_RS08050 Protein ID WP_000323025.1
Coordinates 1620628..1620915 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HQ24_RS08055 Protein ID WP_000534858.1
Coordinates 1620915..1621154 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ24_RS08010 1615652..1615867 - 216 WP_000839590.1 phage lysis protein EssD -
HQ24_RS08015 1616621..1616836 - 216 WP_000066484.1 cold shock-like protein CspB -
HQ24_RS08020 1617137..1617349 + 213 WP_000087756.1 cold shock-like protein CspF -
HQ24_RS26190 1617404..1617493 + 90 WP_120795389.1 hypothetical protein -
HQ24_RS08025 1617771..1618523 - 753 WP_001047135.1 antitermination protein -
HQ24_RS08030 1618537..1619586 - 1050 WP_001265198.1 DUF968 domain-containing protein -
HQ24_RS08035 1619588..1619866 - 279 WP_012304870.1 hypothetical protein -
HQ24_RS08040 1619933..1620184 - 252 WP_000980994.1 hypothetical protein -
HQ24_RS08045 1620401..1620556 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HQ24_RS08050 1620628..1620915 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HQ24_RS08055 1620915..1621154 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HQ24_RS24280 1621179..1621484 + 306 WP_001326990.1 hypothetical protein -
HQ24_RS08060 1621687..1622019 + 333 WP_001301033.1 protein FlxA -
HQ24_RS23555 1622456..1622605 - 150 WP_011443592.1 hypothetical protein -
HQ24_RS08065 1622640..1622918 - 279 Protein_1541 hypothetical protein -
HQ24_RS08070 1622902..1623132 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
HQ24_RS08075 1623216..1623623 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
HQ24_RS08080 1623790..1623945 + 156 WP_000379575.1 DUF1391 family protein -
HQ24_RS08085 1624105..1624323 + 219 WP_001171942.1 hypothetical protein -
HQ24_RS08095 1624891..1625079 + 189 WP_000854559.1 cell division inhibition protein DicB -
HQ24_RS08100 1625076..1625267 + 192 WP_001083297.1 lysis protein YdfD -
HQ24_RS24295 1625360..1626127 + 768 Protein_1548 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1603634..1629197 25563


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T47503 WP_000323025.1 NZ_CP008801:c1620915-1620628 [Escherichia coli KLY]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T47503 NZ_CP008801:c1620915-1620628 [Escherichia coli KLY]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT47503 WP_000534858.1 NZ_CP008801:c1621154-1620915 [Escherichia coli KLY]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT47503 NZ_CP008801:c1621154-1620915 [Escherichia coli KLY]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References