Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3579685..3580316 Replicon chromosome
Accession NZ_CP007037
Organism Shigella flexneri G1663

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag SF2A_RS19410 Protein ID WP_001259384.1
Coordinates 3580041..3580316 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SF2A_RS19405 Protein ID WP_000593555.1
Coordinates 3579685..3580044 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF2A_RS19380 (3575817) 3575817..3576761 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
SF2A_RS19385 (3576758) 3576758..3577591 + 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
SF2A_RS19390 (3577591) 3577591..3578355 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
SF2A_RS19395 (3578352) 3578352..3579158 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
SF2A_RS19400 (3579164) 3579164..3579565 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
SF2A_RS19405 (3579685) 3579685..3580044 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SF2A_RS19410 (3580041) 3580041..3580316 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
SF2A_RS19415 (3580375) 3580375..3581499 - 1125 WP_001216257.1 ABC transporter permease -
SF2A_RS19420 (3581499) 3581499..3584234 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
SF2A_RS19425 (3584231) 3584231..3585298 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3579685..3596782 17097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T45989 WP_001259384.1 NZ_CP007037:c3580316-3580041 [Shigella flexneri G1663]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T45989 NZ_CP007037:c3580316-3580041 [Shigella flexneri G1663]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAGCCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT45989 WP_000593555.1 NZ_CP007037:c3580044-3579685 [Shigella flexneri G1663]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT45989 NZ_CP007037:c3580044-3579685 [Shigella flexneri G1663]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References