Detailed information of TA system TA040423

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2800537..2800746
• Replicon: chromosome
• Accession: NZ_AP025558
• Organism: Clostridioides difficile strain CE91-St50

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: -
• Locus tag: MWL96_RS13030
• Protein ID: WP_003431040.1
• Coordinates: 2800588..2800746 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2800537..2800671 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWL96_RS13000 (2795740)	2795740..2795940	+	201	WP_003431034.1	cold-shock protein	-
MWL96_RS13005 (2796386)	2796386..2797708	+	1323	WP_021427477.1	ATP-binding protein	-
MWL96_RS13010 (2797751)	2797751..2798332	+	582	WP_003431036.1	accessory gene regulator B family protein	-
MWL96_RS13015 (2798350)	2798350..2798511	+	162	WP_003431037.1	cyclic lactone autoinducer peptide	-
MWL96_RS13020 (2798533)	2798533..2799330	-	798	WP_003431038.1	N-acetylmuramoyl-L-alanine amidase	-
MWL96_RS13025 (2799327)	2799327..2799587	-	261	WP_003431039.1	phage holin family protein	-
- (2800537)	2800537..2800671	+	135	NuclAT_0	-	Antitoxin
- (2800537)	2800537..2800671	+	135	NuclAT_0	-	Antitoxin
- (2800537)	2800537..2800671	+	135	NuclAT_0	-	Antitoxin
MWL96_RS13030 (2800588)	2800588..2800746	-	159	WP_003431040.1	hypothetical protein	Toxin
MWL96_RS13035 (2801104)	2801104..2802474	-	1371	WP_003431041.1	cell wall-binding protein Cwp29	-
MWL96_RS13045 (2802909)	2802909..2803295	+	387	WP_231299769.1	hypothetical protein	-
MWL96_RS13050 (2803673)	2803673..2803798	+	126	WP_003431043.1	hypothetical protein	-
MWL96_RS13055 (2804161)	2804161..2804241	-	81	Protein_2517	VOC family protein	-
MWL96_RS13060 (2804281)	2804281..2804949	-	669	WP_003431044.1	VanZ family protein	-
MWL96_RS13065 (2805048)	2805048..2805431	-	384	WP_071599195.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5982.18 Da
• Isoelectric Point: 11.3102

>T40423 WP_003431040.1 NZ_AP025558:c2800746-2800588 [Clostridioides difficile]
MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T40423 NZ_AP025558:c2800746-2800588 [Clostridioides difficile]
ATGGATAATTTTTTACAAGGCATACTAGCAAGTCTATCTGCTAGCTTAATAGTTTATGTAGCTAGCAAACTATTTAGAAA
GCGTAAAAAACCACTCAAAGCGGCAACTAAGAGTGGTTGGGAATTTGATTTAAAAATCAGATTCCATAAAACTAAGTAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT40423 NZ_AP025558:2800537-2800671 [Clostridioides difficile]
AAGAAGAGCTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Antitoxin