Detailed information of TA system TA038862

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): IasE-IsrA/SymE(toxin)
• Location: 760753..761010
• Replicon: chromosome
• Accession: NZ_AP024585
• Organism: Citrobacter amalonaticus strain JCM 1661

Toxin (Protein)

• Gene name: IasE
• Uniprot ID: -
• Locus tag: KI228_RS03750
• Protein ID: WP_224267567.1
• Coordinates: 760753..761010 (+)
• Length: 86 a.a.

Antitoxin (RNA)

Gene name	IsrA
Locus tag	-
Coordinates	760782..761000 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI228_RS03730 (CIAM_07300)	755924..757783	+	1860	WP_054177121.1	bifunctional glutathionylspermidine amidase/synthase	-
KI228_RS24265	758558..758710	-	153	WP_231912847.1	replication initiation protein	-
KI228_RS03740 (CIAM_07330)	759215..759754	+	540	WP_044255706.1	hypothetical protein	-
KI228_RS03745	759922..760780	+	859	Protein_734	IS3 family transposase	-
KI228_RS03750 (CIAM_07360)	760753..761010	+	258	WP_224267567.1	SymE family type I addiction module toxin	Toxin
-	760782..761000	-	219	-	-	Antitoxin
KI228_RS03755 (CIAM_07370)	761061..761381	-	321	WP_061070583.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (11-50)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9414.82 Da
• Isoelectric Point: 8.5042

>T38862 WP_224267567.1 NZ_AP024585:760753-761010 [Citrobacter amalonaticus]
MGRATCSKTHMTTYYSRIPSLHLKGDWLAEAGFDTGASVTVKISEGCLTLIVETDEVRDLRKELYQVKKSMKHIKAGVNN
VVNGD

Nucleotide

• Length: 258 bp

>T38862 NZ_AP024585:760753-761010 [Citrobacter amalonaticus]
ATGGGCAGAGCAACATGCAGTAAAACTCACATGACCACCTATTACTCACGCATTCCCAGTCTGCATCTCAAGGGTGACTG
GCTGGCAGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGATCTCCGAGGGGTGTTTGACTCTGATTGTGGAGA
CAGACGAAGTGAGAGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTAAATAAT
GTGGTGAACGGGGATTAA

Antitoxin

Nucleotide

• Length: 219 bp

>AT38862 NZ_AP024585:c761000-760782 [Citrobacter amalonaticus]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCTCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCCTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCACCCTTGAGATGCAGACTGGGAATGCGTGAGTAATAGGTGGTCATG

Structures

Antitoxin