Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3520055..3520770 Replicon chromosome
Accession NZ_LR878365
Organism Shigella flexneri 3a isolate 83

Toxin (Protein)


Gene name yafO Uniprot ID A0A658YZ27
Locus tag JMV05_RS17980 Protein ID WP_001263492.1
Coordinates 3520055..3520474 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag JMV05_RS17985 Protein ID WP_000554759.1
Coordinates 3520477..3520770 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV05_RS17955 (3515358) 3515358..3516815 + 1458 WP_025759252.1 cytosol nonspecific dipeptidase -
JMV05_RS17960 (3516872) 3516872..3517432 - 561 Protein_3507 peptide chain release factor H -
JMV05_RS17965 (3517548) 3517548..3518592 - 1045 Protein_3508 RNA ligase RtcB family protein -
JMV05_RS17970 (3518838) 3518838..3519290 - 453 WP_001059862.1 GNAT family N-acetyltransferase -
JMV05_RS17980 (3520055) 3520055..3520474 - 420 WP_001263492.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JMV05_RS17985 (3520477) 3520477..3520770 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JMV05_RS17990 (3520822) 3520822..3521877 - 1056 WP_001226172.1 DNA polymerase IV -
JMV05_RS17995 (3521948) 3521948..3522733 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
JMV05_RS18000 (3522705) 3522705..3524417 + 1713 Protein_3515 flagellar biosynthesis protein FlhA -
JMV05_RS18005 (3524634) 3524634..3525131 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gtrB / gmhA/lpcA 3458709..3556366 97657
flank IS/Tn - - 3519324..3519827 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16348.95 Da        Isoelectric Point: 8.0871

>T289456 WP_001263492.1 NZ_LR878365:c3520474-3520055 [Shigella flexneri 3a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFWVMLPTY

Download         Length: 420 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT289456 WP_000554759.1 NZ_LR878365:c3520770-3520477 [Shigella flexneri 3a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A658YZ27

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References