Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1615306..1615832 Replicon chromosome
Accession NZ_LM993812
Organism Escherichia coli strain K-12 substr. HMS174

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECHMS174_RS08000 Protein ID WP_000323025.1
Coordinates 1615306..1615593 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECHMS174_RS08005 Protein ID WP_000534858.1
Coordinates 1615593..1615832 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHMS174_RS07960 1610330..1610545 - 216 WP_000839590.1 phage lysis protein EssD -
ECHMS174_RS07965 1611299..1611514 - 216 WP_000066484.1 cold shock-like protein CspB -
ECHMS174_RS07970 1611815..1612027 + 213 WP_000087756.1 cold shock-like protein CspF -
ECHMS174_RS25355 1612082..1612171 + 90 WP_120795389.1 hypothetical protein -
ECHMS174_RS07975 1612449..1613201 - 753 WP_001047135.1 antitermination protein -
ECHMS174_RS07980 1613215..1614264 - 1050 WP_001393597.1 DUF968 domain-containing protein -
ECHMS174_RS07985 1614266..1614544 - 279 WP_012304870.1 hypothetical protein -
ECHMS174_RS07990 1614611..1614862 - 252 WP_000980994.1 hypothetical protein -
ECHMS174_RS07995 1615079..1615234 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
ECHMS174_RS08000 1615306..1615593 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECHMS174_RS08005 1615593..1615832 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECHMS174_RS23530 1615857..1616162 + 306 WP_001326990.1 hypothetical protein -
ECHMS174_RS08010 1616365..1616697 + 333 WP_001301033.1 protein FlxA -
ECHMS174_RS22810 1617134..1617283 - 150 WP_011443592.1 hypothetical protein -
ECHMS174_RS08015 1617318..1617596 - 279 Protein_1534 hypothetical protein -
ECHMS174_RS08020 1617580..1617810 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
ECHMS174_RS08025 1617894..1618301 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
ECHMS174_RS08030 1618468..1618623 + 156 WP_000379575.1 DUF1391 family protein -
ECHMS174_RS08035 1618783..1619001 + 219 WP_001171942.1 hypothetical protein -
ECHMS174_RS08045 1619569..1619757 + 189 WP_000854559.1 cell division inhibition protein DicB -
ECHMS174_RS08050 1619754..1619912 + 159 WP_045152978.1 DUF1482 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1600962..1621216 20254
- inside Prophage - - 1584764..1621216 36452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T285399 WP_000323025.1 NZ_LM993812:c1615593-1615306 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT285399 WP_000534858.1 NZ_LM993812:c1615832-1615593 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References