Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1362026..1362657 Replicon chromosome
Accession NZ_CP121223
Organism Shigella flexneri 2a strain Sflex 21-42

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag P5F89_RS08795 Protein ID WP_001259384.1
Coordinates 1362382..1362657 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag P5F89_RS08790 Protein ID WP_000593555.1
Coordinates 1362026..1362385 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P5F89_RS08765 (1358158) 1358158..1359102 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
P5F89_RS08770 (1359099) 1359099..1359932 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
P5F89_RS08775 (1359932) 1359932..1360696 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
P5F89_RS08780 (1360693) 1360693..1361499 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
P5F89_RS08785 (1361505) 1361505..1361906 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
P5F89_RS08790 (1362026) 1362026..1362385 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
P5F89_RS08795 (1362382) 1362382..1362657 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
P5F89_RS08800 (1362716) 1362716..1363840 - 1125 WP_001216257.1 ABC transporter permease -
P5F89_RS08805 (1363840) 1363840..1366575 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
P5F89_RS08810 (1366572) 1366572..1367639 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1362026..1381770 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T276094 WP_001259384.1 NZ_CP121223:c1362657-1362382 [Shigella flexneri 2a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT276094 WP_000593555.1 NZ_CP121223:c1362385-1362026 [Shigella flexneri 2a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References