Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3624350..3624981 Replicon chromosome
Accession NC_017328
Organism Shigella flexneri 2002017

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag SFXV_RS19710 Protein ID WP_001259384.1
Coordinates 3624706..3624981 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SFXV_RS19705 Protein ID WP_000593555.1
Coordinates 3624350..3624709 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFXV_RS19680 (3620482) 3620482..3621426 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
SFXV_RS19685 (3621423) 3621423..3622256 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
SFXV_RS19690 (3622256) 3622256..3623020 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
SFXV_RS19695 (3623017) 3623017..3623823 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
SFXV_RS19700 (3623829) 3623829..3624230 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
SFXV_RS19705 (3624350) 3624350..3624709 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SFXV_RS19710 (3624706) 3624706..3624981 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
SFXV_RS19715 (3625040) 3625040..3626164 - 1125 WP_001216257.1 ABC transporter permease -
SFXV_RS19720 (3626164) 3626164..3628899 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
SFXV_RS19725 (3628896) 3628896..3629963 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3624350..3644096 19746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T26551 WP_001259384.1 NC_017328:c3624981-3624706 [Shigella flexneri 2002017]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T26551 NC_017328:c3624981-3624706 [Shigella flexneri 2002017]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT26551 WP_000593555.1 NC_017328:c3624709-3624350 [Shigella flexneri 2002017]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT26551 NC_017328:c3624709-3624350 [Shigella flexneri 2002017]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References