Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yjhX-yjhQ/YjhX-GNAT
Location 4537967..4538781 Replicon chromosome
Accession NZ_CP095083
Organism Escherichia coli strain EC6868

Toxin (Protein)


Gene name yjhX Uniprot ID S1PA82
Locus tag MUK76_RS22155 Protein ID WP_001054376.1
Coordinates 4537967..4538224 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name yjhQ Uniprot ID U9Z4B8
Locus tag MUK76_RS22160 Protein ID WP_001309181.1
Coordinates 4538236..4538781 (+) Length 182 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK76_RS22130 (4533227) 4533227..4534207 + 981 WP_000991438.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
MUK76_RS22135 (4534317) 4534317..4534522 + 206 Protein_4353 HNH endonuclease -
MUK76_RS22140 (4534822) 4534822..4535802 - 981 WP_000019400.1 IS5-like element IS5 family transposase -
MUK76_RS22145 (4535989) 4535989..4537229 - 1241 Protein_4355 helicase YjhR -
MUK76_RS22150 (4537345) 4537345..4537476 + 132 WP_001309182.1 hypothetical protein -
MUK76_RS22155 (4537967) 4537967..4538224 + 258 WP_001054376.1 YjhX family toxin Toxin
MUK76_RS22160 (4538236) 4538236..4538781 + 546 WP_001309181.1 N-acetyltransferase Antitoxin
MUK76_RS22165 (4538837) 4538837..4539583 + 747 WP_000354251.1 class I SAM-dependent methyltransferase -
MUK76_RS22170 (4539752) 4539752..4539970 + 219 Protein_4360 hypothetical protein -
MUK76_RS22175 (4540008) 4540008..4540124 + 117 Protein_4361 VOC family protein -
MUK76_RS22180 (4540369) 4540369..4541490 + 1122 WP_000010829.1 M42 family metallopeptidase -
MUK76_RS22185 (4541487) 4541487..4541765 + 279 WP_000722973.1 PTS sugar transporter subunit IIB SgcB -
MUK76_RS22190 (4541777) 4541777..4543090 + 1314 WP_000460843.1 PTS sugar transporter subunit IIC SgcC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4534822..4535802 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(18-121)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9734.29 Da        Isoelectric Point: 11.0090

>T241231 WP_001054376.1 NZ_CP095083:4537967-4538224 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Download         Length: 258 bp

>T241231 NZ_LT963400:c11706-11407 [Pseudomonas cerasi]
ATGACGACGATAAAACAGACCTCTACCTATATGGCTTGGGAACGCAGGCTTAAAGACCAGAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCTCCAGTAGGACAGGGCGTTAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCCAAGAAACTGGCTGATCAATGGTGGCAAGAATGA

Antitoxin


Download         Length: 182 a.a.        Molecular weight: 19956.90 Da        Isoelectric Point: 6.3277

>AT241231 WP_001309181.1 NZ_CP095083:4538236-4538781 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Download         Length: 546 bp

>AT241231 NZ_LT963400:c11410-11126 [Pseudomonas cerasi]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGTCCTGAAGCAATCGCGATTTTTATCTCTGATGC
CTTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGACCGAGCTTGCGC
GCGAGACAGGTCTGTCTCGAGAGCAGCTTTATCGGTCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGATATGACAGCTCGAATGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PA82

Antitoxin

Source ID Structure
AlphaFold DB U9Z4B8

References