Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symE-rdlD/SymE(toxin)
Location 829860..830272 Replicon chromosome
Accession NZ_CP093368
Organism Escherichia coli strain EV36 mutant 1a

Toxin (Protein)


Gene name symE Uniprot ID U9YSY7
Locus tag L0S95_RS04000 Protein ID WP_000132601.1
Coordinates 829860..830201 (-) Length 114 a.a.

Antitoxin (RNA)


Gene name rdlD
Locus tag -
Coordinates 830196..830272 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0S95_RS03990 (827273) 827273..828319 - 1047 WP_000437621.1 5-methylcytosine-specific restriction endonuclease system specificity protein McrC -
L0S95_RS03995 (828319) 828319..829698 - 1380 WP_000443951.1 5-methylcytosine-specific restriction endonuclease subunit McrB -
L0S95_RS04000 (829860) 829860..830201 - 342 WP_000132601.1 endoribonuclease SymE Toxin
- (830196) 830196..830272 + 77 NuclAT_6 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_6 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_6 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_6 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_7 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_7 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_7 - Antitoxin
- (830196) 830196..830272 + 77 NuclAT_7 - Antitoxin
L0S95_RS04005 (830429) 830429..831823 - 1395 WP_001272447.1 type I restriction-modification system specificity subunit -
L0S95_RS04010 (831820) 831820..833409 - 1590 WP_001063204.1 type I restriction-modification system methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 822775..831823 9048


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12203.02 Da        Isoelectric Point: 8.5012

>T238241 WP_000132601.1 NZ_CP093368:c830201-829860 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Download         Length: 342 bp

>T238241 NZ_LR890286:c5079362-5078979 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 bp

>AT238241 NZ_CP093368:830196-830272 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YSY7

Antitoxin

Download structure file

References