Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 3073243..3073970 | Replicon | chromosome |
| Accession | NC_011601 | ||
| Organism | Escherichia coli O127:H6 str. E2348/69 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | V0YLE2 |
| Locus tag | E2348C_RS16000 | Protein ID | WP_000547555.1 |
| Coordinates | 3073659..3073970 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | E2348C_RS15995 | Protein ID | WP_000126295.1 |
| Coordinates | 3073243..3073662 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E2348C_RS15975 (E2348_C_2977) | 3069541..3070068 | - | 528 | WP_001078777.1 | electron transport protein HydN | - |
| E2348C_RS15980 (E2348_C_2978) | 3070602..3071975 | + | 1374 | WP_000244358.1 | IS4-like element ISEc13 family transposase | - |
| E2348C_RS15985 (E2348_C_2979) | 3072113..3072571 | + | 459 | WP_000526113.1 | IS200/IS605-like element IS200C family transposase | - |
| E2348C_RS15990 (E2348_C_2980) | 3072723..3073151 | + | 429 | WP_000536065.1 | DUF4259 domain-containing protein | - |
| E2348C_RS15995 (E2348_C_2981) | 3073243..3073662 | - | 420 | WP_000126295.1 | helix-turn-helix domain-containing protein | Antitoxin |
| E2348C_RS16000 | 3073659..3073970 | - | 312 | WP_000547555.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| E2348C_RS16005 (E2348_C_2982) | 3074137..3074607 | - | 471 | WP_000132968.1 | hydrogenase maturation peptidase HycI | - |
| E2348C_RS16010 (E2348_C_2983) | 3074600..3075010 | - | 411 | WP_001291918.1 | formate hydrogenlyase assembly protein HycH | - |
| E2348C_RS16015 (E2348_C_2984) | 3075007..3075774 | - | 768 | WP_000067411.1 | formate hydrogenlyase subunit HycG | - |
| E2348C_RS16020 (E2348_C_2985) | 3075774..3076316 | - | 543 | WP_000493801.1 | formate hydrogenlyase subunit HycF | - |
| E2348C_RS16025 (E2348_C_2986) | 3076326..3078035 | - | 1710 | WP_001288134.1 | formate hydrogenlyase subunit HycE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Genomic island | - | - | 3064460..3073970 | 9510 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12439.18 Da Isoelectric Point: 9.5146
>T23261 WP_000547555.1 NC_011601:c3073970-3073659 [Escherichia coli O127:H6 str. E2348/69]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE
Download Length: 312 bp
>T23261 NC_011601:c3073970-3073659 [Escherichia coli O127:H6 str. E2348/69]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15406.38 Da Isoelectric Point: 4.4596
>AT23261 WP_000126295.1 NC_011601:c3073662-3073243 [Escherichia coli O127:H6 str. E2348/69]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMSV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMSV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT23261 NC_011601:c3073662-3073243 [Escherichia coli O127:H6 str. E2348/69]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGTCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTATCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAATAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGTCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTATCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|