Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 19251..19734 Replicon plasmid pXF-Riv16
Accession NZ_CP090317
Organism Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)


Gene name pasB Uniprot ID A0A060HFL1
Locus tag IUD23_RS12090 Protein ID WP_014607314.1
Coordinates 19251..19520 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A060HE94
Locus tag IUD23_RS12095 Protein ID WP_038211605.1
Coordinates 19504..19734 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IUD23_RS12055 (IUD23_12055) 15724..16107 - 384 WP_013087959.1 VirB3 family type IV secretion system protein -
IUD23_RS12060 (IUD23_12060) 16112..16432 - 321 WP_013087960.1 TrbC/VirB2 family protein -
IUD23_RS12065 (IUD23_12065) 16435..16908 - 474 WP_013087961.1 hypothetical protein -
IUD23_RS12070 (IUD23_12070) 16917..17096 - 180 WP_014607310.1 hypothetical protein -
IUD23_RS12075 (IUD23_12075) 17922..18281 + 360 WP_038211603.1 hypothetical protein -
IUD23_RS12080 (IUD23_12080) 18375..19046 - 672 WP_013087963.1 restriction endonuclease -
IUD23_RS12085 (IUD23_12085) 19071..19199 + 129 WP_234056444.1 type II toxin-antitoxin system PemK/MazF family toxin -
IUD23_RS12090 (IUD23_12090) 19251..19520 - 270 WP_014607314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IUD23_RS12095 (IUD23_12095) 19504..19734 - 231 WP_038211605.1 DUF6290 family protein Antitoxin
IUD23_RS12100 (IUD23_12100) 19911..20513 + 603 WP_013087965.1 recombinase family protein -
IUD23_RS12105 (IUD23_12105) 20510..21373 + 864 WP_234056439.1 plasmid replication initiator TrfA -
IUD23_RS12110 (IUD23_12110) 21817..22053 + 237 WP_013087968.1 antitoxin MazE family protein -
IUD23_RS12115 (IUD23_12115) 22050..22377 + 328 Protein_28 type II toxin-antitoxin system PemK/MazF family toxin -
IUD23_RS12120 (IUD23_12120) 22482..22925 - 444 WP_014607318.1 hypothetical protein -
IUD23_RS12125 (IUD23_12125) 22926..23678 - 753 WP_013087971.1 hypothetical protein -
IUD23_RS12130 (IUD23_12130) 23691..23951 - 261 WP_234056440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..25102 25102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-88)

Antitoxin

Protein of unknown function DUF6290

(1-70)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10252.86 Da        Isoelectric Point: 9.9871

>T230437 WP_014607314.1 NZ_CP090317:c19520-19251 [Xylella fastidiosa subsp. morus]
MAWKVELSSLAQKNLDQLDPQIALRVLSFLHERVAPLDDPRSIGESLKGSRLGEFWKYRVGDYRIISSIEDGALRILVVK
IGNRREVYR

Download         Length: 270 bp

>T230437 NZ_CP124898:c2450268-2450152 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8686.80 Da        Isoelectric Point: 4.2465

>AT230437 WP_038211605.1 NZ_CP090317:c19734-19504 [Xylella fastidiosa subsp. morus]
MASVSLRLPDEVTQRLENLAALTGRSKTFYMVEAIREHIDDLEDLYLAERELEAIRSGNSQTVPLEEVMQRYGMEG

Download         Length: 231 bp

>AT230437 NZ_CP124898:2450036-2450241 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060HFL1

Antitoxin

Source ID Structure
AlphaFold DB A0A060HE94

References