Detailed information of TA system TA229960

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 3317115..3317335
• Replicon: chromosome
• Accession: NZ_CP090060
• Organism: Clostridioides difficile strain CDB

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: A0A069AVC0
• Locus tag: LWC40_RS15265
• Protein ID: WP_009901465.1
• Coordinates: 3317174..3317335 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	3317115..3317254 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWC40_RS15240 (3312340)	3312340..3313146	-	807	WP_021361719.1	BRO family protein	-
LWC40_RS15245 (3313272)	3313272..3313442	-	171	WP_021361718.1	ribbon-helix-helix domain-containing protein	-
LWC40_RS15250 (3314235)	3314235..3314411	-	177	WP_021388791.1	hypothetical protein	-
LWC40_RS15255 (3314466)	3314466..3315332	-	867	WP_095908768.1	BRO family protein	-
LWC40_RS15260 (3315451)	3315451..3315639	-	189	WP_003429858.1	hypothetical protein	-
- (3317115)	3317115..3317254	+	140	NuclAT_4	-	Antitoxin
- (3317115)	3317115..3317254	+	140	NuclAT_4	-	Antitoxin
- (3317115)	3317115..3317254	+	140	NuclAT_5	-	Antitoxin
- (3317115)	3317115..3317254	+	140	NuclAT_6	-	Antitoxin
LWC40_RS15265 (3317174)	3317174..3317335	-	162	WP_009901465.1	hypothetical protein	Toxin
LWC40_RS15270 (3317656)	3317656..3318096	-	441	WP_021362035.1	phage XkdN-like family protein	-
LWC40_RS15275 (3318101)	3318101..3318358	-	258	WP_021362036.1	hypothetical protein	-
LWC40_RS15280 (3318412)	3318412..3318882	-	471	WP_021362037.1	phage tail tube protein	-
LWC40_RS15285 (3318907)	3318907..3320217	-	1311	WP_021362038.1	phage tail sheath family protein	-
LWC40_RS15290 (3320218)	3320218..3320394	-	177	WP_009901506.1	hypothetical protein	-
LWC40_RS15295 (3320387)	3320387..3320824	-	438	WP_009901504.1	hypothetical protein	-
LWC40_RS15300 (3320817)	3320817..3321242	-	426	WP_021362039.1	HK97 gp10 family phage protein	-
LWC40_RS15305 (3321242)	3321242..3321589	-	348	WP_021362040.1	hypothetical protein	-
LWC40_RS15310 (3321589)	3321589..3321933	-	345	WP_021362041.1	phage head-tail connector protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6034.28 Da
• Isoelectric Point: 11.0253

>T229960 WP_009901465.1 NZ_CP090060:c3317335-3317174 [Clostridioides difficile]
MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELGFKIKFRKFK

Nucleotide

• Length: 162 bp

>T229960 NZ_CP124651:1980419-1981006 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Nucleotide

• Length: 140 bp

>AT229960 NZ_CP090060:3317115-3317254 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGCGGTAGAGTGAAGTTCATAATTTTAAAAAATATAAATTATTTAAATTTGCGGAACTT
TATTTTAAAACCAAGTTCCCAGCCACTTTTACTCTTGCCACGAGTAGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVC0

Antitoxin