Detailed information of TA system TA229953

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2786154..2786363
• Replicon: chromosome
• Accession: NZ_CP090060
• Organism: Clostridioides difficile strain CDB

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: LWC40_RS12990
• Protein ID: WP_004454815.1
• Coordinates: 2786205..2786363 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2786154..2786288 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWC40_RS12975 (2781581)	2781581..2783209	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
LWC40_RS12980 (2783330)	2783330..2784325	+	996	WP_003431031.1	asparaginase	-
LWC40_RS12985 (2784514)	2784514..2784798	-	285	WP_224213953.1	BlaI/MecI/CopY family transcriptional regulator	-
- (2786154)	2786154..2786288	+	135	NuclAT_1	-	Antitoxin
- (2786154)	2786154..2786288	+	135	NuclAT_1	-	Antitoxin
- (2786154)	2786154..2786288	+	135	NuclAT_2	-	Antitoxin
LWC40_RS12990 (2786205)	2786205..2786363	-	159	WP_004454815.1	hypothetical protein	Toxin
LWC40_RS12995 (2786722)	2786722..2788092	-	1371	WP_021381153.1	cell wall-binding protein Cwp29	-
LWC40_RS13005 (2788528)	2788528..2788614	+	87	WP_231298880.1	hypothetical protein	-
LWC40_RS19470 (2788836)	2788836..2789225	+	390	WP_021361937.1	tyrosine-type recombinase/integrase	-
LWC40_RS19475 (2789276)	2789276..2789416	+	141	WP_021405537.1	phage integrase family protein	-
LWC40_RS13015 (2789733)	2789733..2790401	-	669	WP_021361938.1	VanZ family protein	-
LWC40_RS19480 (2790500)	2790500..2790631	-	132	WP_021361939.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T229953 WP_004454815.1 NZ_CP090060:c2786363-2786205 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T229953 NZ_CP124649:2535531-2536106 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Nucleotide

• Length: 135 bp

>AT229953 NZ_CP090060:2786154-2786288 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin