Detailed information of TA system TA229950

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2543086..2543288
• Replicon: chromosome
• Accession: NZ_CP090060
• Organism: Clostridioides difficile strain CDB

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: LWC40_RS11870
• Protein ID: WP_004454589.1
• Coordinates: 2543136..2543288 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2543086..2543215 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWC40_RS11840 (2538520)	2538520..2538984	+	465	WP_011861514.1	hypothetical protein	-
LWC40_RS11845 (2539312)	2539312..2539473	-	162	WP_004454578.1	hypothetical protein	-
LWC40_RS11850 (2539525)	2539525..2539920	-	396	WP_011861515.1	hypothetical protein	-
LWC40_RS11855 (2539973)	2539973..2540338	-	366	WP_011861516.1	Bro-N domain-containing protein	-
LWC40_RS11860 (2540458)	2540458..2540646	-	189	WP_021369679.1	virulence factor Srl	-
LWC40_RS11865 (2542019)	2542019..2542942	-	924	WP_011861517.1	SHOCT domain-containing protein	-
- (2543086)	2543086..2543215	+	130	NuclAT_2	-	Antitoxin
- (2543086)	2543086..2543215	+	130	NuclAT_2	-	Antitoxin
- (2543086)	2543086..2543215	+	130	NuclAT_3	-	Antitoxin
LWC40_RS11870 (2543136)	2543136..2543288	-	153	WP_004454589.1	hypothetical protein	Toxin
LWC40_RS11875 (2543562)	2543562..2543885	-	324	WP_009897482.1	DUF6054 family protein	-
LWC40_RS11880 (2543921)	2543921..2544175	-	255	WP_004454592.1	hypothetical protein	-
LWC40_RS11885 (2544611)	2544611..2545129	-	519	Protein_2267	RNA-guided endonuclease TnpB family protein	-
LWC40_RS11890 (2545419)	2545419..2545794	-	376	Protein_2268	BlaI/MecI/CopY family transcriptional regulator	-
LWC40_RS11895 (2545899)	2545899..2546276	-	378	WP_011861518.1	BlaI/MecI/CopY family transcriptional regulator	-
LWC40_RS11900 (2547080)	2547080..2547574	+	495	WP_011861519.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
LWC40_RS11905 (2547935)	2547935..2548133	+	199	Protein_2271	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T229950 WP_004454589.1 NZ_CP090060:c2543288-2543136 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T229950 NZ_CP124649:c143688-143407 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT229950 NZ_CP090060:2543086-2543215 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin