224364

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4464829..4465355 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LMF34_RS21410 Protein ID WP_000323025.1
Coordinates 4465068..4465355 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LMF34_RS21405 Protein ID WP_000534858.1
Coordinates 4464829..4465068 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS21375 (4460399) 4460399..4460681 + 283 Protein_4170 SAM-dependent methyltransferase -
LMF34_RS21380 (4461159) 4461159..4461580 + 422 Protein_4171 hypothetical protein -
LMF34_RS21385 (4462235) 4462235..4463257 + 1023 Protein_4172 ISNCY family transposase -
LMF34_RS21390 (4463378) 4463378..4463527 + 150 WP_011443592.1 protein YdfW -
LMF34_RS21395 (4463964) 4463964..4464296 - 333 WP_001301033.1 FlxA-like family protein -
LMF34_RS21400 (4464499) 4464499..4464804 - 306 WP_001326990.1 protein YdfV -
LMF34_RS21405 (4464829) 4464829..4465068 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LMF34_RS21410 (4465068) 4465068..4465355 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LMF34_RS21415 (4465427) 4465427..4465582 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LMF34_RS21420 (4465799) 4465799..4466050 + 252 WP_000980994.1 protein Rem -
LMF34_RS21425 (4466117) 4466117..4466395 + 279 WP_012304870.1 hypothetical protein -
LMF34_RS21430 (4466397) 4466397..4467446 + 1050 WP_001265198.1 DUF968 domain-containing protein -
LMF34_RS21435 (4467460) 4467460..4468212 + 753 WP_001047135.1 antitermination protein -
LMF34_RS21440 (4468490) 4468490..4468579 - 90 WP_120795389.1 hypothetical protein -
LMF34_RS21445 (4468634) 4468634..4468846 - 213 WP_248422963.1 cold shock-like protein CspF -
LMF34_RS21450 (4469147) 4469147..4469362 + 216 WP_000066484.1 cold shock-like protein CspB -
LMF34_RS21455 (4469726) 4469726..4469896 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
LMF34_RS21460 (4470116) 4470116..4470331 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4438760..4500996 62236
flank IS/Tn - - 4459347..4460327 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T224364 WP_000323025.1 NZ_CP086678:4465068-4465355 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T224364 NZ_CP120315:836177-836575 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT224364 WP_000534858.1 NZ_CP086678:4464829-4465068 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT224364 NZ_CP120315:835950-836177 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References