Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4464829..4465355 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LMF34_RS21410 Protein ID WP_000323025.1
Coordinates 4465068..4465355 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LMF34_RS21405 Protein ID WP_000534858.1
Coordinates 4464829..4465068 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS21375 (4460399) 4460399..4460681 + 283 Protein_4170 SAM-dependent methyltransferase -
LMF34_RS21380 (4461159) 4461159..4461580 + 422 Protein_4171 hypothetical protein -
LMF34_RS21385 (4462235) 4462235..4463257 + 1023 Protein_4172 ISNCY family transposase -
LMF34_RS21390 (4463378) 4463378..4463527 + 150 WP_011443592.1 protein YdfW -
LMF34_RS21395 (4463964) 4463964..4464296 - 333 WP_001301033.1 FlxA-like family protein -
LMF34_RS21400 (4464499) 4464499..4464804 - 306 WP_001326990.1 protein YdfV -
LMF34_RS21405 (4464829) 4464829..4465068 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LMF34_RS21410 (4465068) 4465068..4465355 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LMF34_RS21415 (4465427) 4465427..4465582 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LMF34_RS21420 (4465799) 4465799..4466050 + 252 WP_000980994.1 protein Rem -
LMF34_RS21425 (4466117) 4466117..4466395 + 279 WP_012304870.1 hypothetical protein -
LMF34_RS21430 (4466397) 4466397..4467446 + 1050 WP_001265198.1 DUF968 domain-containing protein -
LMF34_RS21435 (4467460) 4467460..4468212 + 753 WP_001047135.1 antitermination protein -
LMF34_RS21440 (4468490) 4468490..4468579 - 90 WP_120795389.1 hypothetical protein -
LMF34_RS21445 (4468634) 4468634..4468846 - 213 WP_248422963.1 cold shock-like protein CspF -
LMF34_RS21450 (4469147) 4469147..4469362 + 216 WP_000066484.1 cold shock-like protein CspB -
LMF34_RS21455 (4469726) 4469726..4469896 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
LMF34_RS21460 (4470116) 4470116..4470331 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4438760..4500996 62236
flank IS/Tn - - 4459347..4460327 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T224364 WP_000323025.1 NZ_CP086678:4465068-4465355 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T224364 NZ_CP120315:836177-836575 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT224364 WP_000534858.1 NZ_CP086678:4464829-4465068 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT224364 NZ_CP120315:835950-836177 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References