Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1648691..1649217 Replicon chromosome
Accession NZ_CP084899
Organism Escherichia coli strain W3110-P

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LHV60_RS08130 Protein ID WP_000323025.1
Coordinates 1648691..1648978 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LHV60_RS08135 Protein ID WP_000534858.1
Coordinates 1648978..1649217 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LHV60_RS08080 (1643715) 1643715..1643930 - 216 WP_000839590.1 phage lysis protein EssD -
LHV60_RS08085 (1644150) 1644150..1644320 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
LHV60_RS08090 (1644684) 1644684..1644899 - 216 WP_000066484.1 cold shock-like protein CspB -
LHV60_RS08095 (1645200) 1645200..1645412 + 213 WP_000087756.1 cold shock-like protein CspF -
LHV60_RS08100 (1645467) 1645467..1645556 + 90 WP_120795389.1 hypothetical protein -
LHV60_RS08105 (1645834) 1645834..1646586 - 753 WP_001047135.1 antitermination protein -
LHV60_RS08110 (1646600) 1646600..1647649 - 1050 WP_001393597.1 DUF968 domain-containing protein -
LHV60_RS08115 (1647651) 1647651..1647929 - 279 WP_012304870.1 hypothetical protein -
LHV60_RS08120 (1647996) 1647996..1648247 - 252 WP_000980994.1 protein Rem -
LHV60_RS08125 (1648464) 1648464..1648619 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LHV60_RS08130 (1648691) 1648691..1648978 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LHV60_RS08135 (1648978) 1648978..1649217 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LHV60_RS08140 (1649242) 1649242..1649547 + 306 WP_001326990.1 protein YdfV -
LHV60_RS08145 (1649750) 1649750..1650082 + 333 WP_001301033.1 FlxA-like family protein -
LHV60_RS08150 (1650519) 1650519..1650668 - 150 WP_011443592.1 protein YdfW -
LHV60_RS08155 (1650703) 1650703..1650981 - 279 Protein_1594 hypothetical protein -
LHV60_RS08160 (1650965) 1650965..1651195 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
LHV60_RS08165 (1651279) 1651279..1651686 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
LHV60_RS08170 (1651853) 1651853..1652008 + 156 WP_000379575.1 DUF1391 family protein -
LHV60_RS22615 (1652010) 1652010..1652138 + 129 WP_000344964.1 protein YdfB -
LHV60_RS08175 (1652168) 1652168..1652386 + 219 WP_001171942.1 protein YdfC -
LHV60_RS08180 (1652954) 1652954..1653142 + 189 WP_000854559.1 cell division inhibition protein DicB -
LHV60_RS08185 (1653139) 1653139..1653330 + 192 WP_001083297.1 lysis protein YdfD -
LHV60_RS08190 (1653423) 1653423..1654190 + 768 Protein_1602 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1634347..1656053 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T220085 WP_000323025.1 NZ_CP084899:c1648978-1648691 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T220085 NZ_CP116929:c1734343-1733966 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT220085 WP_000534858.1 NZ_CP084899:c1649217-1648978 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT220085 NZ_CP116929:c1734801-1734433 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References