Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 298535..299166 Replicon chromosome
Accession NZ_CP084879
Organism Shigella flexneri strain CMCC51574

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag LGV73_RS01385 Protein ID WP_001259384.1
Coordinates 298535..298810 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LGV73_RS01390 Protein ID WP_000593555.1
Coordinates 298807..299166 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LGV73_RS01370 (293553) 293553..294620 + 1068 WP_000361470.1 HlyD family secretion protein -
LGV73_RS01375 (294617) 294617..297352 + 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
LGV73_RS01380 (297352) 297352..298476 + 1125 WP_001216257.1 ABC transporter permease -
LGV73_RS01385 (298535) 298535..298810 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
LGV73_RS01390 (298807) 298807..299166 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LGV73_RS01395 (299286) 299286..299687 - 402 WP_001190069.1 nickel-responsive transcriptional regulator NikR -
LGV73_RS01400 (299693) 299693..300499 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
LGV73_RS01405 (300496) 300496..301260 - 765 WP_001136248.1 nickel import ATP-binding protein NikD -
LGV73_RS01410 (301260) 301260..302093 - 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
LGV73_RS01415 (302090) 302090..303034 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 278131..299166 21035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T219774 WP_001259384.1 NZ_CP084879:298535-298810 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T219774 NZ_CP116701:2099965-2101206 [Agrobacterium fabacearum]
ATGACAACCATCTACTACGAGACCTTGCCTGTCGCCCATCTGACTTTTGAAGGCGAATGGAGGTTGGACTATGATCCGGG
CTGGGAAGCACGCCGCCCGGCCTTTCCGGTGTCGCTTACCATGCCGCTGCGTTCCGGATTCGTGGGTGCAGCGAAGCTGC
TTCCCTGGCTTGCCAATCTGCTTCCGGAAACCCATCTTGCGGAGATCGGTCAGCGCCTCAAGGTTTCCCCGCAAGATATT
GTCGGTCTCCTTGCTCATATCGGACGTGACACGGCGGGGGCACTGTCGATCGGGGAGCCGCGGAAAGCTGGCGTCAACCT
CCGGCCCGTGCCGGACGAGCAGGCGCTGGAGCGTATTCTCAATGAACTTCCTGCCAAACCCTTTCTGGTCGGAGAGCGCG
GTGTTTCCATGTCGCTTGCTGGCGTCCAGGAGAAACTGCCGGTGTTTGCTGACGGGAACGGCCGTATTTCAGTTCCGGTC
GACGGGATGCCATCCACGCATATTCTAAAGCCGGATACGAAACGCCTTGCGGGGAGCGTGGAGAACGAAGCCTTCTGCCT
GTCGCTTGCGCGTGCCTGCGGCCTGGATGCCGCCGAGGCGACGATCGGAGTAGCTGGAAAGCGACGTTACCTTCTCGTGA
AACGCTATGATCGCTTTACCGACGCTCAAGGCGAGATCCGCAGGCTGCATCAGGAGGATCTTTGCCAGTTAACGGGTTAC
TTCCCCTCGCAGAAATACGAGCGATCCTCGACCGGCCCCGGCGTGACCATGAAGATGATGTTTGACGCCGTCAGCAATCT
TGTTTCGCCTGGCGAGCGGTTGAAACTTCTCGATGCGGTGATCTTCAATGTCCTGATCTGCAATTCGGATTCGCATGCCA
AAAATTACTCTATCCTGATTGGTGCCGGTGGGTCCGCGAAGATGGCTCCACTCTATGACCTGATGTGCGCTGCCGTTTAT
CGCCGGGTCGACCAGAGCCTGCCGCAGGGGATTTCCGGTCGGTTCCATGCGCCTGACGTGCGGCGCGCCGACTGGCAGGC
GCTGGCTGATGACATTGGACTGAGCGGCGCATCGACACTGAAACGGGTGGAGGAGCTTGCAAAACTGGTCAGCGTCGCAT
GCGATGAGGTGGCACCATATATCGTCGCCCTCTCCAGCGATCCGACGAGGATTCTTGAGCGAATTACTCACAGCGTTCAA
AAGCGGTGCCGGCGAATTCAGGAGCAGATGCATGATCGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT219774 WP_000593555.1 NZ_CP084879:298807-299166 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT219774 NZ_CP116701:2099696-2099968 [Agrobacterium fabacearum]
ATGTTCAGGTCCGCGCGTGATTTCGGCGCTGCTATCAAGGAGAGACGCAAAGCACTTGGCTGGACGCAAACCGGGCTTGC
TGCCCGTTCCGGAACGGGGGAACGCTTCATCGTCGAGCTTGAATCGGGCAAGCCGAGTTGCCAGCTCGAAAAAGCCCTGA
TCGTCGCACGCGCAGTTGGAATCGATCTTGGCGATCTCAAGACGGTTCAGACTGTGCAAGTCGCGCCGGACGATGACCTC
GGCTTTCTGCCGATGTTTGGTGCTGATCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References