217894

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2233623..2234149 Replicon chromosome
Accession NZ_CP083990
Organism Escherichia coli strain EC2648-Vir2

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LCH23_RS10790 Protein ID WP_000323025.1
Coordinates 2233862..2234149 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LCH23_RS10785 Protein ID WP_000534858.1
Coordinates 2233623..2233862 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCH23_RS10730 (2228650) 2228650..2229417 - 768 Protein_2106 exonuclease -
LCH23_RS10735 (2229510) 2229510..2229701 - 192 WP_001083297.1 lysis protein YdfD -
LCH23_RS10740 (2229698) 2229698..2229886 - 189 WP_000854559.1 cell division inhibition protein DicB -
LCH23_RS10745 (2230454) 2230454..2230672 - 219 WP_001171942.1 protein YdfC -
LCH23_RS23855 (2230702) 2230702..2230830 - 129 WP_000344964.1 protein YdfB -
LCH23_RS10750 (2230832) 2230832..2230987 - 156 WP_000379575.1 DUF1391 family protein -
LCH23_RS10755 (2231154) 2231154..2231561 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
LCH23_RS10760 (2231645) 2231645..2231875 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
LCH23_RS10765 (2231859) 2231859..2232137 + 279 Protein_2114 protein YdfX -
LCH23_RS10770 (2232172) 2232172..2232321 + 150 WP_011443592.1 protein YdfW -
LCH23_RS10775 (2232758) 2232758..2233090 - 333 WP_001301033.1 FlxA-like family protein -
LCH23_RS10780 (2233293) 2233293..2233598 - 306 WP_001326990.1 protein YdfV -
LCH23_RS10785 (2233623) 2233623..2233862 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LCH23_RS10790 (2233862) 2233862..2234149 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LCH23_RS10795 (2234221) 2234221..2234376 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LCH23_RS10800 (2234593) 2234593..2234844 + 252 WP_000980994.1 protein Rem -
LCH23_RS10805 (2234911) 2234911..2235189 + 279 WP_012304870.1 hypothetical protein -
LCH23_RS10810 (2235191) 2235191..2236240 + 1050 WP_001265198.1 DUF968 domain-containing protein -
LCH23_RS10815 (2236254) 2236254..2237006 + 753 WP_001047135.1 antitermination protein -
LCH23_RS10820 (2237284) 2237284..2237373 - 90 WP_120795389.1 hypothetical protein -
LCH23_RS10825 (2237428) 2237428..2237640 - 213 WP_000087756.1 cold shock-like protein CspF -
LCH23_RS10830 (2237941) 2237941..2238156 + 216 WP_000066484.1 cold shock-like protein CspB -
LCH23_RS10835 (2238520) 2238520..2238690 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
LCH23_RS10840 (2238910) 2238910..2239125 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2226787..2250280 23493


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T217894 WP_000323025.1 NZ_CP083990:2233862-2234149 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T217894 NZ_CP114893:3027234-3027488 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT217894 WP_000534858.1 NZ_CP083990:2233623-2233862 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT217894 NZ_CP114893:3026986-3027237 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References