Detailed information of TA system TA216884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2448615..2448829
• Replicon: chromosome
• Accession: NZ_CP083616
• Organism: Clostridioides difficile strain FDAARGOS_1528

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: LA362_RS10900
• Protein ID: WP_003429855.1
• Coordinates: 2448668..2448829 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	2448615..2448748 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA362_RS10875 (2444317)	2444317..2445120	-	804	WP_021377655.1	ORF6N domain-containing protein	-
LA362_RS10880 (2445364)	2445364..2445567	+	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
LA362_RS10885 (2445980)	2445980..2446156	-	177	WP_009894378.1	hypothetical protein	-
LA362_RS10890 (2446219)	2446219..2447046	-	828	WP_021368246.1	hypothetical protein	-
LA362_RS10895 (2447170)	2447170..2447343	-	174	WP_018112722.1	hypothetical protein	-
- (2448165)	2448165..2448270	+	106	NuclAT_3	-	-
- (2448165)	2448165..2448270	+	106	NuclAT_3	-	-
- (2448165)	2448165..2448270	+	106	NuclAT_4	-	-
- (2448165)	2448165..2448270	+	106	NuclAT_5	-	-
- (2448615)	2448615..2448748	+	134	NuclAT_1	-	Antitoxin
- (2448615)	2448615..2448748	+	134	NuclAT_1	-	Antitoxin
- (2448615)	2448615..2448748	+	134	NuclAT_1	-	Antitoxin
- (2448615)	2448615..2448748	+	134	NuclAT_2	-	Antitoxin
LA362_RS10900 (2448668)	2448668..2448829	-	162	WP_003429855.1	hypothetical protein	Toxin
LA362_RS10905 (2449170)	2449170..2449610	-	441	WP_003429853.1	phage portal protein	-
LA362_RS10910 (2449682)	2449682..2450152	-	471	WP_003429851.1	phage tail tube protein	-
LA362_RS10915 (2450169)	2450169..2451479	-	1311	WP_021368245.1	phage tail sheath family protein	-
LA362_RS10920 (2451481)	2451481..2451657	-	177	WP_021362376.1	hypothetical protein	-
LA362_RS10925 (2451650)	2451650..2452087	-	438	WP_021362375.1	hypothetical protein	-
LA362_RS10930 (2452436)	2452436..2453242	-	807	WP_021358994.1	hypothetical protein	-
LA362_RS10935 (2453352)	2453352..2453642	-	291	WP_021358995.1	HK97 gp10 family phage protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T216884 WP_003429855.1 NZ_CP083616:c2448829-2448668 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T216884 NZ_CP113503:c4110417-4110103 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Nucleotide

• Length: 134 bp

>AT216884 NZ_CP083616:2448615-2448748 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin