Detailed information of TA system TA216881

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1580343..1580545
• Replicon: chromosome
• Accession: NZ_CP083616
• Organism: Clostridioides difficile strain FDAARGOS_1528

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: LA362_RS06840
• Protein ID: WP_004454589.1
• Coordinates: 1580343..1580495 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	1580416..1580545 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA362_RS06810 (1576061)	1576061..1576555	-	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
LA362_RS06815 (1577360)	1577360..1577737	+	378	WP_021364350.1	BlaI/MecI/CopY family transcriptional regulator	-
LA362_RS20590 (1577842)	1577842..1578216	+	375	Protein_1353	BlaI/MecI/CopY family transcriptional regulator	-
LA362_RS06825 (1578506)	1578506..1579024	+	519	Protein_1354	transposase	-
LA362_RS06830 (1579456)	1579456..1579710	+	255	WP_004454592.1	hypothetical protein	-
LA362_RS06835 (1579746)	1579746..1580069	+	324	WP_021364628.1	DUF6054 family protein	-
LA362_RS06840 (1580343)	1580343..1580495	+	153	WP_004454589.1	hypothetical protein	Toxin
- (1580416)	1580416..1580545	-	130	NuclAT_2	-	Antitoxin
- (1580416)	1580416..1580545	-	130	NuclAT_2	-	Antitoxin
- (1580416)	1580416..1580545	-	130	NuclAT_3	-	Antitoxin
LA362_RS06845 (1580689)	1580689..1581612	+	924	WP_021364626.1	SHOCT domain-containing protein	-
LA362_RS06850 (1582751)	1582751..1582903	+	153	WP_009897477.1	hypothetical protein	-
LA362_RS06855 (1583023)	1583023..1583388	+	366	WP_009897471.1	Bro-N domain-containing protein	-
LA362_RS06860 (1583385)	1583385..1583837	+	453	WP_009897470.1	hypothetical protein	-
LA362_RS06865 (1583889)	1583889..1584050	+	162	WP_004454578.1	hypothetical protein	-
LA362_RS06870 (1584225)	1584225..1584350	+	126	Protein_1363	hypothetical protein	-
LA362_RS06875 (1584379)	1584379..1584843	-	465	WP_004454576.1	hypothetical protein	-
LA362_RS06880 (1584979)	1584979..1585296	-	318	WP_009897465.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T216881 WP_004454589.1 NZ_CP083616:1580343-1580495 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T216881 NZ_CP113503:3601928-3602146 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT216881 NZ_CP083616:c1580545-1580416 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin