Detailed information of TA system TA216878

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1336202..1336411
• Replicon: chromosome
• Accession: NZ_CP083616
• Organism: Clostridioides difficile strain FDAARGOS_1528

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: LA362_RS05710
• Protein ID: WP_004454815.1
• Coordinates: 1336202..1336360 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	1336277..1336411 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA362_RS05685 (1332163)	1332163..1332831	+	669	WP_009897711.1	VanZ family protein	-
LA362_RS05690 (1333149)	1333149..1333421	-	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
LA362_RS20515 (1333951)	1333951..1334142	-	192	Protein_1133	site-specific integrase	-
LA362_RS05705 (1334473)	1334473..1335843	+	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
LA362_RS05710 (1336202)	1336202..1336360	+	159	WP_004454815.1	hypothetical protein	Toxin
- (1336277)	1336277..1336411	-	135	NuclAT_0	-	Antitoxin
- (1336277)	1336277..1336411	-	135	NuclAT_0	-	Antitoxin
- (1336277)	1336277..1336411	-	135	NuclAT_0	-	Antitoxin
LA362_RS05715 (1337603)	1337603..1337851	+	249	WP_231298879.1	BlaI/MecI/CopY family transcriptional regulator	-
LA362_RS05720 (1338040)	1338040..1339035	-	996	WP_003431031.1	asparaginase	-
LA362_RS05725 (1339156)	1339156..1340784	-	1629	WP_004454813.1	aspartate 4-decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T216878 WP_004454815.1 NZ_CP083616:1336202-1336360 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T216878 NZ_CP113503:2609522-2609800 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Nucleotide

• Length: 135 bp

>AT216878 NZ_CP083616:c1336411-1336277 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin