Detailed information of TA system TA215294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4439680..4440494
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K8B73_RS21355
• Protein ID: WP_001054376.1
• Coordinates: 4439680..4439937 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K8B73_RS21360
• Protein ID: WP_001309181.1
• Coordinates: 4439949..4440494 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS21335 (4434940)	4434940..4435920	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K8B73_RS26425 (4436030)	4436030..4436235	+	206	Protein_4194	HNH endonuclease	-
K8B73_RS21340 (4436535)	4436535..4437515	-	981	WP_000019400.1	IS5-like element IS5 family transposase	-
K8B73_RS21345 (4437702)	4437702..4438942	-	1241	Protein_4196	helicase YjhR	-
K8B73_RS26430 (4439058)	4439058..4439189	+	132	WP_001309182.1	hypothetical protein	-
K8B73_RS21355 (4439680)	4439680..4439937	+	258	WP_001054376.1	YjhX family toxin	Toxin
K8B73_RS21360 (4439949)	4439949..4440494	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K8B73_RS21365 (4440550)	4440550..4441296	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K8B73_RS21370 (4441465)	4441465..4441683	+	219	Protein_4201	hypothetical protein	-
K8B73_RS21375 (4441721)	4441721..4441837	+	117	Protein_4202	VOC family protein	-
K8B73_RS21380 (4442082)	4442082..4443203	+	1122	WP_000010829.1	M42 family metallopeptidase	-
K8B73_RS21385 (4443200)	4443200..4443478	+	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K8B73_RS21390 (4443490)	4443490..4444803	+	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4430975..4448718	17743
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 IS/Tn TA006348 3666255..3667235 0.003 View NC_000913 Escherichia coli str. K-12 substr. MG1655 IS/Tn TA006364 3652182..3653162 0.003	flank	IS/Tn	-	-	4436535..4437515	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T215294 WP_001054376.1 NZ_CP082827:4439680-4439937 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T215294 NZ_CP110620:c732251-731937 [Pasteurella multocida]
ATGCTCGATATAATTGAAACAACAGTATTTAATAAATGGCTTAAAGAGCTAAAGGATTTGTCGGCTAAAGCGGCTATTCT
TGCACGTATTAGTCGCGCTAAAAGTGGTAATTTTGGTGACCATAAATCAGTTGGTGATGGTTTGTATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATACAAAGATGTAACCTATTTGCTAATTTGTGGCGGTGATAAATCT
ACTCAGAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAGAAAGAGGAGATAAATGTATGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT215294 WP_001309181.1 NZ_CP082827:4439949-4440494 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT215294 NZ_CP110620:c731940-731650 [Pasteurella multocida]
ATGAGTAAAAAAGTAGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAATTTTAGAAACAGGTGACACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCGGCAAAAACAGGATTAGGAAGAGAAAGTCTCTATAAAACATTATCACACGGAAGTAAGCCGCGTTTTGATACTATA
ATGAAAATTACGCAAGCACTAGGTATTAAACTTGTCCCAACGCATATCTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8