Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 5654..6255 Replicon plasmid unnamed3
Accession NZ_CP081888
Organism Escherichia coli strain WW2

Toxin (Protein)


Gene name doc Uniprot ID Q47172
Locus tag K5O11_RS24560 Protein ID WP_001694510.1
Coordinates 5875..6255 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag K5O11_RS24555 Protein ID WP_001190712.1
Coordinates 5654..5875 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O11_RS24545 (2716) 2716..3918 - 1203 WP_222563294.1 restriction endonuclease subunit S -
K5O11_RS24550 (3915) 3915..5471 - 1557 WP_222563295.1 type I restriction-modification system subunit M -
K5O11_RS24555 (5654) 5654..5875 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K5O11_RS24560 (5875) 5875..6255 + 381 WP_001694510.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K5O11_RS24565 (6260) 6260..6439 + 180 WP_000113018.1 hypothetical protein -
K5O11_RS24570 (6467) 6467..6745 + 279 Protein_6 pdcB -
K5O11_RS24575 (6750) 6750..7163 + 414 Protein_7 integrase core domain-containing protein -
K5O11_RS24580 (7113) 7113..7448 - 336 WP_169329198.1 type I deoxyribonuclease HsdR -
K5O11_RS24585 (7658) 7658..8638 - 981 WP_001567628.1 IS5-like element IS5 family transposase -
K5O11_RS24590 (8882) 8882..10285 + 1404 WP_001373486.1 S-methylmethionine permease -
K5O11_RS24595 (10272) 10272..11204 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..103282 103282
flank IS/Tn - - 7658..8638 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.35 Da        Isoelectric Point: 5.1514

>T213635 WP_001694510.1 NZ_CP081888:5875-6255 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T213635 NZ_CP109744:2085838-2086245 [Citrobacter portucalensis]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTGTTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTATTGATGGATGGCCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGTATGATGCTACGCTTGCGATCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCAGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AAGGGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT213635 WP_001190712.1 NZ_CP081888:5654-5875 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT213635 NZ_CP109744:2085591-2085857 [Citrobacter portucalensis]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGAGCAGCTGGAACAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A737M8C8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References