Detailed information of TA system TA212616

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2826415..2826624
• Replicon: chromosome
• Accession: NZ_CP081021
• Organism: Clostridioides difficile strain G89

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: -
• Locus tag: K2215_RS13210
• Protein ID: WP_003431040.1
• Coordinates: 2826466..2826624 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2826415..2826549 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2215_RS13180 (2821618)	2821618..2821818	+	201	WP_003431034.1	cold-shock protein	-
K2215_RS13185 (2822264)	2822264..2823586	+	1323	WP_021427477.1	ATP-binding protein	-
K2215_RS13190 (2823629)	2823629..2824210	+	582	WP_003431036.1	accessory gene regulator B family protein	-
K2215_RS13195 (2824228)	2824228..2824389	+	162	WP_003431037.1	cyclic lactone autoinducer peptide	-
K2215_RS13200 (2824411)	2824411..2825208	-	798	WP_003431038.1	N-acetylmuramoyl-L-alanine amidase	-
K2215_RS13205 (2825205)	2825205..2825465	-	261	WP_003431039.1	phage holin family protein	-
- (2826415)	2826415..2826549	+	135	NuclAT_1	-	Antitoxin
- (2826415)	2826415..2826549	+	135	NuclAT_1	-	Antitoxin
- (2826415)	2826415..2826549	+	135	NuclAT_2	-	Antitoxin
K2215_RS13210 (2826466)	2826466..2826624	-	159	WP_003431040.1	hypothetical protein	Toxin
K2215_RS13215 (2826982)	2826982..2828352	-	1371	WP_003431041.1	cell wall-binding protein Cwp29	-
K2215_RS13220 (2828787)	2828787..2829173	+	387	WP_231299769.1	hypothetical protein	-
K2215_RS13225 (2829551)	2829551..2829676	+	126	WP_003431043.1	hypothetical protein	-
K2215_RS13230 (2830039)	2830039..2830119	-	81	Protein_2531	VOC family protein	-
K2215_RS13235 (2830159)	2830159..2830827	-	669	WP_003431044.1	VanZ family protein	-
K2215_RS13240 (2830926)	2830926..2831309	-	384	WP_071599195.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5982.18 Da
• Isoelectric Point: 11.3102

>T212616 WP_003431040.1 NZ_CP081021:c2826624-2826466 [Clostridioides difficile]
MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T212616 NZ_CP107297:4252429-4252812 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT212616 NZ_CP081021:2826415-2826549 [Clostridioides difficile]
AAGAAGAGCTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Antitoxin