Detailed information of TA system TA212612

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1764200..1764414
• Replicon: chromosome
• Accession: NZ_CP081021
• Organism: Clostridioides difficile strain G89

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: K2215_RS08315
• Protein ID: WP_003429855.1
• Coordinates: 1764200..1764361 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1764281..1764414 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2215_RS08270 (1759540)	1759540..1759773	+	234	WP_003433536.1	hypothetical protein	-
K2215_RS08275 (1759783)	1759783..1760184	+	402	WP_009888836.1	hypothetical protein	-
K2215_RS08280 (1760178)	1760178..1760525	+	348	WP_003433540.1	hypothetical protein	-
K2215_RS08285 (1760525)	1760525..1760950	+	426	WP_003433542.1	HK97 gp10 family phage protein	-
K2215_RS08290 (1760943)	1760943..1761380	+	438	WP_003433544.1	hypothetical protein	-
K2215_RS08295 (1761373)	1761373..1761549	+	177	WP_003433548.1	hypothetical protein	-
K2215_RS08300 (1761550)	1761550..1762860	+	1311	WP_003433550.1	phage tail sheath family protein	-
K2215_RS08305 (1762877)	1762877..1763347	+	471	WP_003429851.1	phage tail tube protein	-
K2215_RS08310 (1763419)	1763419..1763859	+	441	WP_003429853.1	phage portal protein	-
K2215_RS08315 (1764200)	1764200..1764361	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1764281)	1764281..1764414	-	134	NuclAT_0	-	Antitoxin
- (1764281)	1764281..1764414	-	134	NuclAT_0	-	Antitoxin
- (1764281)	1764281..1764414	-	134	NuclAT_0	-	Antitoxin
- (1764281)	1764281..1764414	-	134	NuclAT_1	-	Antitoxin
- (1764759)	1764759..1764807	-	49	NuclAT_4	-	-
- (1764759)	1764759..1764807	-	49	NuclAT_4	-	-
- (1764759)	1764759..1764807	-	49	NuclAT_5	-	-
- (1764759)	1764759..1764807	-	49	NuclAT_6	-	-
K2215_RS08320 (1765509)	1765509..1766261	+	753	WP_021387708.1	SHOCT domain-containing protein	-
K2215_RS08325 (1766565)	1766565..1766738	+	174	WP_003433557.1	hypothetical protein	-
K2215_RS08330 (1766862)	1766862..1767689	+	828	WP_003433559.1	hypothetical protein	-
K2215_RS08335 (1767738)	1767738..1767914	+	177	WP_003433561.1	hypothetical protein	-
K2215_RS08340 (1768345)	1768345..1768905	+	561	WP_021387709.1	ribosomal protein L7/L12	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T212612 WP_003429855.1 NZ_CP081021:1764200-1764361 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T212612 NZ_CP107297:c3296493-3296086 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Nucleotide

• Length: 134 bp

>AT212612 NZ_CP081021:c1764414-1764281 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin