Detailed information of TA system TA212605

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2698575..2698789
• Replicon: chromosome
• Accession: NZ_CP081019
• Organism: Clostridioides difficile strain CD099

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: K2218_RS12545
• Protein ID: WP_003429855.1
• Coordinates: 2698628..2698789 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	2698575..2698708 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2218_RS12520 (K2218_12500)	2694084..2694644	-	561	WP_021387709.1	ribosomal protein L7/L12	-
K2218_RS12525 (K2218_12505)	2695075..2695251	-	177	WP_003433561.1	hypothetical protein	-
K2218_RS12530 (K2218_12510)	2695300..2696127	-	828	WP_003433559.1	hypothetical protein	-
K2218_RS12535 (K2218_12515)	2696251..2696424	-	174	WP_003433557.1	hypothetical protein	-
K2218_RS12540 (K2218_12520)	2696728..2697480	-	753	WP_021387708.1	SHOCT domain-containing protein	-
-	2698575..2698708	+	134	-	-	Antitoxin
K2218_RS12545 (K2218_12525)	2698628..2698789	-	162	WP_003429855.1	hypothetical protein	Toxin
K2218_RS12550 (K2218_12530)	2699130..2699570	-	441	WP_003429853.1	phage portal protein	-
K2218_RS12555 (K2218_12535)	2699642..2700112	-	471	WP_003429851.1	phage tail tube protein	-
K2218_RS12560 (K2218_12540)	2700129..2701439	-	1311	WP_003433550.1	phage tail sheath family protein	-
K2218_RS12565 (K2218_12545)	2701440..2701616	-	177	WP_003433548.1	hypothetical protein	-
K2218_RS12570 (K2218_12550)	2701609..2702046	-	438	WP_003433544.1	hypothetical protein	-
K2218_RS12575 (K2218_12555)	2702039..2702464	-	426	WP_003433542.1	HK97 gp10 family phage protein	-
K2218_RS12580 (K2218_12560)	2702464..2702811	-	348	WP_003433540.1	hypothetical protein	-
K2218_RS12585 (K2218_12565)	2702805..2703206	-	402	WP_009888836.1	hypothetical protein	-
K2218_RS12590 (K2218_12570)	2703216..2703449	-	234	WP_003433536.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2664386..2731007	66621

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T212605 WP_003429855.1 NZ_CP081019:c2698789-2698628 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T212605 NZ_CP107292:212781-213092 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 134 bp

>AT212605 NZ_CP081019:2698575-2698708 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin