Detailed information of TA system TA212602

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1637647..1637856
• Replicon: chromosome
• Accession: NZ_CP081019
• Organism: Clostridioides difficile strain CD099

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: -
• Locus tag: K2218_RS07675
• Protein ID: WP_003431040.1
• Coordinates: 1637647..1637805 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	1637722..1637856 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2218_RS07645 (K2218_07615)	1632962..1633345	+	384	WP_071599195.1	helix-turn-helix transcriptional regulator	-
K2218_RS07650 (K2218_07620)	1633444..1634112	+	669	WP_003431044.1	VanZ family protein	-
K2218_RS07655 (K2218_07625)	1634152..1634232	+	81	Protein_1416	VOC family protein	-
K2218_RS07660 (K2218_07630)	1634595..1634720	-	126	WP_003431043.1	hypothetical protein	-
K2218_RS07665 (K2218_07635)	1635098..1635484	-	387	WP_231299769.1	hypothetical protein	-
K2218_RS07670 (K2218_07645)	1635919..1637289	+	1371	WP_003431041.1	cell wall-binding protein Cwp29	-
K2218_RS07675 (K2218_07650)	1637647..1637805	+	159	WP_003431040.1	hypothetical protein	Toxin
-	1637722..1637856	-	135	-	-	Antitoxin
K2218_RS07680 (K2218_07655)	1638675..1638935	+	261	WP_003431039.1	phage holin family protein	-
K2218_RS07685 (K2218_07660)	1638932..1639729	+	798	WP_003431038.1	N-acetylmuramoyl-L-alanine amidase	-
K2218_RS07690 (K2218_07665)	1639751..1639912	-	162	WP_003431037.1	cyclic lactone autoinducer peptide	-
K2218_RS07695 (K2218_07670)	1639930..1640511	-	582	WP_003431036.1	accessory gene regulator B family protein	-
K2218_RS07700 (K2218_07675)	1640554..1641876	-	1323	WP_021427477.1	ATP-binding protein	-
K2218_RS07705 (K2218_07680)	1642322..1642522	-	201	WP_003431034.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1635098..1643687	8589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5982.18 Da
• Isoelectric Point: 11.3102

>T212602 WP_003431040.1 NZ_CP081019:1637647-1637805 [Clostridioides difficile]
MDNFLQGILASLSASLIVYVASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T212602 NZ_CP107291:c5443350-5442967 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 135 bp

>AT212602 NZ_CP081019:c1637856-1637722 [Clostridioides difficile]
AAGAAGAGCTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Antitoxin