Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4183739..4184370 Replicon chromosome
Accession NC_004741
Organism Shigella flexneri 2a str. 2457T

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag S_RS22250 Protein ID WP_001259384.1
Coordinates 4183739..4184014 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822PD91
Locus tag S_RS22255 Protein ID WP_000593553.1
Coordinates 4184011..4184370 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S_RS22235 4178757..4179824 + 1068 WP_000361470.1 HlyD family secretion protein -
S_RS22240 4179821..4182556 + 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
S_RS22245 4182556..4183680 + 1125 WP_001216257.1 ABC transporter permease -
S_RS22250 4183739..4184014 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
S_RS22255 4184011..4184370 + 360 WP_000593553.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
S_RS22260 4184490..4184891 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
S_RS22265 4184897..4185703 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
S_RS22270 4185700..4186464 - 765 WP_001136253.1 nickel import ATP-binding protein NikD -
S_RS22275 4186464..4187297 - 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
S_RS22280 4187294..4188238 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4163334..4184370 21036


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T20951 WP_001259384.1 NC_004741:4183739-4184014 [Shigella flexneri 2a str. 2457T]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T20951 NC_004741:4183739-4184014 [Shigella flexneri 2a str. 2457T]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13474.34 Da        Isoelectric Point: 4.9169

>AT20951 WP_000593553.1 NC_004741:4184011-4184370 [Shigella flexneri 2a str. 2457T]
MIKLKTPNSMEIAEQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT20951 NC_004741:4184011-4184370 [Shigella flexneri 2a str. 2457T]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGAGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A822PD91

References